| Distinct cellular properties of identified dopaminergic and GABAergic neurons in the mouse ventral tegmental area. | |
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MedLine Citation:
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PMID: 21646409 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The midbrain ventral tegmental area (VTA) contains neurons largely with either a dopaminergic (DAergic) or GABAergic phenotype. Physiological and pharmacological properties of DAergic neurons have been determined using tyrosine hydroxylase (TH) immunohistochemistry but many properties overlap with non-DAergic neurons presumed to be GABAergic. This study examined properties of GABAergic neurons, non-GABAergic neurons and TH-immunopositive neurons in VTA of GAD67-GFP knock-in mice. Ninety-eight per cent of VTA neurons were either GAD-GFP or TH positive,with the latter being five times more abundant. During cell-attached patch-clamp recordings, GAD-GFP neurons fired brief action potentials that could be completely distinguished from those of non-GFP neurons. Pharmacologically, the μ-opioid agonist DAMGO inhibited firing of action potentials in 92% of GAD-GFP neurons but had no effect in non-GFP neurons. By contrast, dopamine invariably inhibited action potentials in non-GFP neurons but only did so in 8% of GAD-GFP neurons. During whole-cell recordings, the narrower width of action potential in GAD-GFP neurons was also evident but there was considerable overlap with non-GFP neurons. GAD-GFP neurons invariably failed to exhibit the potassium-mediated slow depolarizing potential during injection of positive current that was present in all non-GFP neurons. Under voltage-clamp the cationic current, I(h), was found in both types of neurons with considerable overlap in both amplitude and kinetics. These distinct cellular properties may thus be used to confidently discriminate GABAergic and DAergic neurons in VTA during in vitro electrophysiological recordings. |
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Authors:
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Billy Chieng; Yael Azriel; Sarasa Mohammadi; MacDonald J Christie |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-06-06 |
Journal Detail:
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Title: The Journal of physiology Volume: 589 ISSN: 1469-7793 ISO Abbreviation: J. Physiol. (Lond.) Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-08-02 Completed Date: 2012-04-17 Revised Date: 2012-09-25 |
Medline Journal Info:
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Nlm Unique ID: 0266262 Medline TA: J Physiol Country: England |
Other Details:
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Languages: eng Pagination: 3775-87 Citation Subset: IM |
Affiliation:
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Brain and Mind Research Institute, University of Sydney, Level 6, Building F, 94 Mallett Street, M02F, Camperdown, NSW 2050, Australia. chin.chieng@sydney.edu.au |
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| MeSH Terms | |
Descriptor/Qualifier:
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Action Potentials
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drug effects Animals Dopamine / metabolism Dopaminergic Neurons / cytology*, metabolism Electrophysiology / methods Enkephalin, Ala(2)-MePhe(4)-Gly(5)- / pharmacology GABAergic Neurons / cytology*, metabolism Gene Knock-In Techniques / methods Glutamate Decarboxylase / metabolism Green Fluorescent Proteins / metabolism Immunohistochemistry / methods Lysine / analogs & derivatives, metabolism Male Mice Patch-Clamp Techniques / methods Potassium / metabolism Tyrosine 3-Monooxygenase / metabolism Ventral Tegmental Area / cytology*, drug effects, metabolism |
| Chemical | |
Reg. No./Substance:
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100929-53-1/Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; 147336-22-9/Green Fluorescent Proteins; 56-87-1/Lysine; 576-19-2/biocytin; 7440-09-7/Potassium; EC 1.14.16.2/Tyrosine 3-Monooxygenase; EC 4.1.1.15/Glutamate Decarboxylase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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