| Distinct alterations in phospholipid metabolism in brains of apolipoprotein E-deficient mice. | |
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MedLine Citation:
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PMID: 10533050 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Apolipoprotein E (ApoE) is the major brain lipoprotein and plays an important role in lipid transport. ApoE-deficient mice whose apoE gene has been knocked out have distinct cognitive and neurochemical deficits, and their recovery from brain injury is impaired. In the present study we examined the possibility that the neuronal derangements of apoE-deficient mice are related to impairments in their phospholipid metabolism. This was performed by comparison of the phospholipid, fatty acid, and cholesterol compositions of distinct membranal brain fractions of apoE-deficient and control mice. Analysis of the microsomal membrane fraction P(3) revealed that, in apoE-deficient mice, these membranes contain significantly lower levels of phosphatidylcholine (PC) than those of control mice. This effect was specific to PC and thus resulted in a twofold decrease of the PC to phosphatidylethanolamine (PE) ratio in apoE-deficient mice compared to the corresponding control ratio. In contrast, the cholesterol levels of the microsomal membranes of the two mice were similar, and the fatty acid composition of their PC was unchanged. There were, however, changes in the fatty acid composition of PE and phosphatidylserine (PS), which resulted in a lower ratio of polyunsaturated to saturated fatty acids in PE and in a higher ratio in apoE-deficient mice compared to the corresponding control values. These effects were specific to the microsomal fraction P(3) and were not observed with the brain subcellular membrane fraction P(2), which is composed mainly of plasma and mitochondrial membranes and whose phospholipid, fatty acid, and cholesterol levels were similar in apoE-deficient and control mice. These findings show that apoE deficiency results in specific and intracellular compartment-dependent changes in phospholipid metabolism, which may play an important role in mediating the neuronal effects of apoE. |
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Authors:
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L Lomnitski; L Oron; D Sklan; D M Michaelson |
Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of neuroscience research Volume: 58 ISSN: 0360-4012 ISO Abbreviation: J. Neurosci. Res. Publication Date: 1999 Nov |
Date Detail:
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Created Date: 1999-12-22 Completed Date: 1999-12-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7600111 Medline TA: J Neurosci Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 586-92 Citation Subset: IM |
Copyright Information:
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Copyright 1999 Wiley-Liss, Inc. |
Affiliation:
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Department of Neurobiochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apolipoproteins E / deficiency* Brain Chemistry / physiology* Cholesterol / metabolism Fatty Acids / chemistry, metabolism Male Membranes / chemistry, metabolism Mice Microsomes / chemistry, metabolism Mitochondria / chemistry, metabolism Nerve Tissue Proteins / analysis, metabolism Phospholipids / metabolism* Subcellular Fractions / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Apolipoproteins E; 0/Fatty Acids; 0/Nerve Tissue Proteins; 0/Phospholipids; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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