| Distinct Sulfonation Activities in Resveratrol-Sensitive and -Insensitive Human Glioblastoma Cells. | |
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MedLine Citation:
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PMID: 22540632 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Glioblastoma multiforme/GBM cells show different responses to resveratrol due to certain unknown reasons. Our data from human medulloblastoma/MB cells and primarily cultured rat brain cells revealed a reverse correlation of sulfonation activity with resveratrol sensitivities, providing a cue to addressing the underlying mechanisms of the variable sensitivities of GBM cells to resveratrol. In this study, we found U251 cells were sensitive and LN229 cells were insensitive to resveratrol. Thus these two cell lines were taken as comparable models for elucidating the influence of sulfonation activities in resveratrol sensitivity. High performance liquid chromatography/HPLC showed identical resveratrol metabolic pattern in both cell lines. Liquid chromatography coupled with tandem mass spectrum/LC/MS and high resolution mass spectrometry/HRMS analyses further demonstrated that resveratrol monosulfate generated by sulfotransferases/SULTs was the major metabolite of human GBM cells. The levels of brain-associated SULTs (SULT1A1, 1C2 and 4A1) expression in U251 cells were lower than that in LN229 cells, suggesting the opposite relationship of SULT-mediated sulfonation activity with high intracellular resveratrol bioavailability and resveratrol-sensitivity of human GBM cells. Furthermore, Immunohistochemical/IHC staining revealed the reduction of three brain-associated SULTs expression in 72.8%, 47.5% and 66.3% of astrocytomas, respectively. Therefore, the levels of brain associated SULTs and sulfonation activity mediated by them would be important parameters for evaluating the potential response of human GBM cells to resveratrol and may have translational values in personalized treatment of GBMs to resveratrol. |
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Authors:
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Zheng Sun; Hong Li; Xiao-Hong Shu; Hui Shi; Xiao-Yan Chen; Qing-You Kong; Mo-Li Wu; Jia Liu |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-4-27 |
Journal Detail:
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Title: The FEBS journal Volume: - ISSN: 1742-4658 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-4-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101229646 Medline TA: FEBS J Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Journal compilation © 2012 Federation of European Biochemical Societies. |
Affiliation:
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Liaoning Laboratory of Cancer Genomics and Department of Cell Biology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China Department of Medicinal Chemistry, College of Pharmacy, Dalian Medical University, Dalian, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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