Document Detail

Distinct Golgi populations of phosphatidylinositol 4-phosphate regulated by phosphatidylinositol 4-kinases.
MedLine Citation:
PMID:  15634669     Owner:  NLM     Status:  MEDLINE    
Phosphatidylinositol 4-phosphate (PI4P) regulates biosynthetic membrane traffic at multiple steps and differentially affects the surface delivery of apically and basolaterally destined proteins in polarized cells. Two phosphatidylinositol 4-kinases (PI4Ks) have been localized to the Golgi complex in mammalian cells, type III PI4Kbeta (PI4KIIIbeta) and type II PI4Kalpha (PI4KIIalpha). Here we report that PI4KIIIbeta and PI4KIIalpha localize to discrete subcompartments of the Golgi complex in Madin-Darby canine kidney (MDCK) cells. PI4KIIIbeta was enriched in early Golgi compartments, whereas PI4KIIalpha colocalized with markers of the trans-Golgi network (TGN). To understand the temporal and spatial control of PI4P generation across the Golgi complex, we quantitated the steady state distribution of a fluorescent PI4P-binding domain relative to cis/medial Golgi and TGN markers in transiently transfected MDCK cells. The density of the signal from this PI4P reporter was roughly 2-fold greater in the early Golgi compartments compared with that of the TGN. Furthermore, this ratio could be modulated in vivo by overexpression of catalytically inactive PI4KIIIbeta and PI4KIIalpha or in vitro by the PI4KIIIbeta inhibitor wortmannin. Our data suggest that both PI4KIIIbeta and PI4KIIalpha contribute to the compartmental regulation of PI4P synthesis within the Golgi complex. We discuss our results with respect to the kinetic effects of modulating PI4K activity on polarized biosynthetic traffic in MDCK cells.
Kelly M Weixel; Anna Blumental-Perry; Simon C Watkins; Meir Aridor; Ora A Weisz
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.     Date:  2005-01-05
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  280     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-03-14     Completed Date:  2005-04-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  10501-8     Citation Subset:  IM    
Renal-Electrolyte Division, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA.
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MeSH Terms
1-Phosphatidylinositol 4-Kinase / metabolism*
Adenoviridae / genetics,  metabolism
Androstadienes / pharmacology
Blood Proteins / chemistry
Cell Line
Cell Membrane / metabolism
Enzyme Inhibitors / pharmacology
Golgi Apparatus / metabolism*
Green Fluorescent Proteins / metabolism
Microscopy, Confocal
Microscopy, Fluorescence
Phosphatidylinositol Phosphates / metabolism*
Phosphoproteins / chemistry
Plasmids / metabolism
Protein Binding
Protein Structure, Tertiary
trans-Golgi Network / metabolism
Grant Support
Reg. No./Substance:
0/Androstadienes; 0/Blood Proteins; 0/Enzyme Inhibitors; 0/Phosphatidylinositol Phosphates; 0/Phosphoproteins; 0/phosphatidylinositol 4-phosphate; 0/platelet protein P47; 147336-22-9/Green Fluorescent Proteins; 19545-26-7/wortmannin; EC 4-Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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