Document Detail

Distinct DNA methylation patterns associated with active and inactive centromeres of the maize B chromosome.
MedLine Citation:
PMID:  21518739     Owner:  NLM     Status:  MEDLINE    
Centromeres are determined by poorly understood epigenetic mechanisms. Centromeres can be activated or inactivated without changing the underlying DNA sequences. However, virtually nothing is known about the epigenetic transition of a centromere from an active to an inactive state because of the lack of examples of the same centromere exhibiting alternative forms and being distinguishable from other centromeres. The centromere of the supernumerary B chromosome of maize provides such an opportunity because its functional core can be cytologically tracked, and an inactive version of the centromere is available. We developed a DNA fiber-based technique that can be used to assess the levels of cytosine methylation associated with repetitive DNA sequences. We report that DNA sequences in the normal B centromere exhibit hypomethylation. This methylation pattern is not affected by the genetic background or structural rearrangement of the B chromosome, but is slightly changed when the B chromosome is transferred to oat as an addition chromosome. In contrast, an inactive version of this same centromere exhibits hypermethylation, indicating that the inactive centromere was modified into a different epigenetic state at the DNA level.
Dal-Hoe Koo; Fangpu Han; James A Birchler; Jiming Jiang
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-04-25
Journal Detail:
Title:  Genome research     Volume:  21     ISSN:  1549-5469     ISO Abbreviation:  Genome Res.     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-06-02     Completed Date:  2011-09-26     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  9518021     Medline TA:  Genome Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  908-14     Citation Subset:  IM    
Department of Horticulture, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
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MeSH Terms
Centromere / genetics*
Chromosomes, Plant / genetics*,  metabolism
Cytosine Nucleotides / metabolism
DNA Methylation / genetics*
In Situ Hybridization, Fluorescence / methods
Microscopy, Fluorescence
Zea mays / genetics*
Reg. No./Substance:
0/Cytosine Nucleotides; 0/Indoles; 47165-04-8/DAPI

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