| Distension enterogenesis: increasing the size and function of small intestine. | |
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MedLine Citation:
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PMID: 16567190 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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INTRODUCTION: The purpose of this study is to evaluate the feasibility of using saline infusion to lengthen small bowel while preserving intestinal enzymatic function. METHODS: Male Sprague-Dawley rats had a 3-cm jejunal segment taken out of continuity. A catheter was inserted in the proximal end, and the distal end was oversewn. Continuous infusion of saline into the isolated jejunal segment was started 2 weeks postoperatively. Segments were harvested 1 week later. Segment weights and lengths were measured preoperatively and at the time of harvest. Histology of harvested segments was performed. Alkaline phosphatase (ALP) and lactase assays were performed. Comparisons were made with normal jejunum from control animals. RESULTS: A 32% increase in length was achieved with saline distension of small intestine. The segment weight to length ratio was significantly increased by saline distension; however, the total protein-to-weight ratio was unchanged. Specific activities of ALP and lactase were not affected by saline distension. Because of the increased length and weight of the distended jejunal segments, total segment activities for both enzymes were significantly increased. CONCLUSIONS: Saline infusion appears to be a viable method for increasing small intestinal length without compromising enzymatic function. This phenomenon may provide a new method for the treatment of patients with short bowel syndrome in the future, and further study is warranted. |
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Authors:
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Devin P Puapong; Benjamin M Wu; Mandy M Lam; James B Atkinson; James C Y Dunn |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of pediatric surgery Volume: 41 ISSN: 1531-5037 ISO Abbreviation: J. Pediatr. Surg. Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-03-28 Completed Date: 2006-10-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0052631 Medline TA: J Pediatr Surg Country: United States |
Other Details:
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Languages: eng Pagination: 763-7 Citation Subset: IM |
Affiliation:
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Department of Surgery, Kaiser Permanente Los Angeles Medical Center, Los Angeles, CA 90027, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Intestine, Small / growth & development*, physiology* Male Organ Size Rats Rats, Sprague-Dawley Tissue Expansion / methods* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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