Document Detail

Distance determination in human ubiquitin by pulsed double electron-electron resonance and double quantum coherence ESR methods.
MedLine Citation:
PMID:  17046296     Owner:  NLM     Status:  MEDLINE    
Recently, distance measurements by pulsed ESR (electron spin resonance) have been obtained using pulsed DEER (double electron-electron resonance) and DQC (double quantum coherence) in SDSL (site directed spin labeling) proteins. These methods can observe long range dipole interactions (15-80A). We applied these methods to human ubiquitin proteins. The distance between the 20th and the 35th cysteine was estimated in doubly spin labeled human ubiquitin. Pulsed DEER requires two microwave sources. However, a phase cycle is not usually required in this method. On the other hand, DQC-ESR at X-band ( approximately 9GHz) can acquire a large echo signal by using pulses of short duration and high power, but this method has an ESEEM (electron spin echo envelope modulation) problem. We used a commercial pulsed ESR spectrometer and compared these two methods.
H Hara; T Tenno; M Shirakawa
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Publication Detail:
Type:  Journal Article     Date:  2006-10-13
Journal Detail:
Title:  Journal of magnetic resonance (San Diego, Calif. : 1997)     Volume:  184     ISSN:  1090-7807     ISO Abbreviation:  J. Magn. Reson.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2007-01-12     Completed Date:  2007-03-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9707935     Medline TA:  J Magn Reson     Country:  United States    
Other Details:
Languages:  eng     Pagination:  78-84     Citation Subset:  IM    
ESR Division, Bruker Biospin K.K., Ibaraki 305-0051, Japan.
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MeSH Terms
Crystallography / methods*
Protein Conformation
Quantum Theory
Signal Processing, Computer-Assisted*
Spin Labels
Ubiquitin / chemistry*,  ultrastructure*
Reg. No./Substance:
0/Spin Labels; 0/Ubiquitin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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