| Distance determination in human ubiquitin by pulsed double electron-electron resonance and double quantum coherence ESR methods. | |
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MedLine Citation:
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PMID: 17046296 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recently, distance measurements by pulsed ESR (electron spin resonance) have been obtained using pulsed DEER (double electron-electron resonance) and DQC (double quantum coherence) in SDSL (site directed spin labeling) proteins. These methods can observe long range dipole interactions (15-80A). We applied these methods to human ubiquitin proteins. The distance between the 20th and the 35th cysteine was estimated in doubly spin labeled human ubiquitin. Pulsed DEER requires two microwave sources. However, a phase cycle is not usually required in this method. On the other hand, DQC-ESR at X-band ( approximately 9GHz) can acquire a large echo signal by using pulses of short duration and high power, but this method has an ESEEM (electron spin echo envelope modulation) problem. We used a commercial pulsed ESR spectrometer and compared these two methods. |
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Authors:
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H Hara; T Tenno; M Shirakawa |
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Publication Detail:
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Type: Journal Article Date: 2006-10-13 |
Journal Detail:
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Title: Journal of magnetic resonance (San Diego, Calif. : 1997) Volume: 184 ISSN: 1090-7807 ISO Abbreviation: J. Magn. Reson. Publication Date: 2007 Jan |
Date Detail:
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Created Date: 2007-01-12 Completed Date: 2007-03-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9707935 Medline TA: J Magn Reson Country: United States |
Other Details:
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Languages: eng Pagination: 78-84 Citation Subset: IM |
Affiliation:
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ESR Division, Bruker Biospin K.K., Ibaraki 305-0051, Japan. Hideyuki.hara@bruker-biospin.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Algorithms* Crystallography / methods* Electrons Humans Protein Conformation Quantum Theory Signal Processing, Computer-Assisted* Spin Labels Ubiquitin / chemistry*, ultrastructure* |
| Chemical | |
Reg. No./Substance:
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0/Spin Labels; 0/Ubiquitin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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