Document Detail

Dissociation of frog olfactory epithelium with N-ethylmaleimide.
MedLine Citation:
PMID:  6975646     Owner:  NLM     Status:  MEDLINE    
Treatment of frog olfactory epithelium with 8 mM N-ethylmaleimide for 2 min results in extensive dissociation of the epithelium. The resulting cell suspension contains single olfactory receptor neurons, sustentacular cells, respiratory epithelial cells, and cells of Bowman's glands. The cells in suspension exhibit the same morphologies seen in histological sections of intact epithelium.
S J Kleene; R C Gesteland
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Brain research     Volume:  229     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  1981 Dec 
Date Detail:
Created Date:  1982-02-22     Completed Date:  1982-02-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  536-40     Citation Subset:  IM    
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MeSH Terms
Cell Separation / methods*
Epithelial Cells
Ethylmaleimide / pharmacology*
Neurons / drug effects,  ultrastructure
Olfactory Mucosa / drug effects,  innervation*
Rana pipiens
Grant Support
Reg. No./Substance:

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