Document Detail

Dissimilar properties of vaccenic versus elaidic acid in beta-oxidation activities and gene regulation in rat liver cells.
MedLine Citation:
PMID:  20508999     Owner:  NLM     Status:  MEDLINE    
Vaccenic acid (trans-11-C(18:1)) chemically resembles elaidic acid (trans-9-C(18:1)) which is assumed to increase the risk of cardiovascular diseases, and thus could exert similar effects. Possible different oxidation rates of vaccenic versus elaidic acid were checked in muscles and liver, and through related gene expression in normal rat liver cells. In hepatic mitochondria, carnitine palmitoyltransferase (CPT) I exhibited comparable activity rates with both trans-isomers. CPT II activity was 30% greater (P < 0.05) with vaccenic than with elaidic acid as nonesterified fatty acids (NEFAs) or acyl-CoAs. Activity of the first beta-oxidation step was similar between the isomers in all the tissue slices and liver extracts assayed. Respiration rates were comparable with both trans-isomers as NEFAs in various liver extracts, but were 30% greater (P < 0.05) with vaccenoyl-CoA than with elaidoyl-CoA in liver mitochondria. Vaccenic acid was oxidised 25% more (P < 0.05) by liver peroxisomes than elaidic acid. In hepatocytes cultured with trans- and corresponding cis-C(18:1) isomers, gene expression of CPT I, hydroxyacyl-CoA dehydrogenase and hydroxymethylglutaryl-CoA synthase was at least 100% increased (P < 0.05), but was unchanged with vaccenic acid, relative to controls. In conclusion, the position and geometry of the double bonds in acyl chains are suggested to confer on vaccenic and elaidic acid specific biochemical properties that might differently affect their fates in tissues.
Zhen-Yu Du; Pascal Degrace; Joseph Gresti; Olivier Loreau; Pierre Clouet
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-28
Journal Detail:
Title:  Lipids     Volume:  45     ISSN:  1558-9307     ISO Abbreviation:  Lipids     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-08     Completed Date:  2010-11-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  581-91     Citation Subset:  IM    
Faculté des Sciences Gabriel, UMR 866, INSERM-UB, Equipe Physiopathologie des dyslipidémies, Université de Bourgogne, 21000, Dijon, France.
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MeSH Terms
Acyl Coenzyme A
Carnitine O-Palmitoyltransferase / genetics,  metabolism
Fatty Acids, Nonesterified / metabolism
Gene Expression Regulation*
Liver / metabolism*
Oleic Acid / chemistry,  metabolism*
Oleic Acids / chemistry,  metabolism*
Rats, Wistar
Reg. No./Substance:
0/Acyl Coenzyme A; 0/Fatty Acids, Nonesterified; 0/Oleic Acids; 112-79-8/elaidic acid; 112-80-1/Oleic Acid; 143-25-9/11-octadecenoic acid; EC O-Palmitoyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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