Document Detail

Dissemination of Cronobacter spp. (Enterobacter sakazakii) in a powdered milk protein manufacturing facility.
MedLine Citation:
PMID:  18641152     Owner:  NLM     Status:  MEDLINE    
The microbial contamination of air filters and possible links to contaminated product in a powdered milk protein-processing facility were investigated. Over a 10-month period, seven air filters, the environment, and powdered product were analyzed for the presence of Cronobacter spp. The effects of air filter installation, maintenance, and subsequent dissemination of Cronobacter were investigated. A total of 30 isolates were characterized by pulsed-field gel electrophoresis (PFGE). PFGE revealed the presence of three clonal populations distributed throughout the manufacturing site. This study highlights the need for proper installation of air filters to limit the dissemination of microorganisms into processing sites.
N Mullane; B Healy; J Meade; P Whyte; P G Wall; S Fanning
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-18
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  74     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-26     Completed Date:  2008-10-23     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5913-7     Citation Subset:  IM    
Centre for Food Safety, School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
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MeSH Terms
Bacterial Typing Techniques
Cluster Analysis
Cronobacter sakazakii / classification*,  genetics,  isolation & purification*
DNA Fingerprinting
DNA, Bacterial / genetics
Electrophoresis, Gel, Pulsed-Field
Environmental Microbiology*
Food Contamination*
Food Microbiology*
Milk Proteins
Reg. No./Substance:
0/DNA, Bacterial; 0/Milk Proteins

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