Document Detail

Dissection of the embryonic brain using photoactivated gene expression.
MedLine Citation:
PMID:  18683638     Owner:  NLM     Status:  MEDLINE    
The Drosophila brain is generated by a complex series of morphogenetic movements. To better understand brain development and to provide a guide for experimental manipulation of brain progenitors, we created a fate map using photoactivated gene expression to mark cells originating within specific mitotic domains and time-lapse microscopy to dynamically monitor their progeny. We show that mitotic domains 1, 5, 9, 20 and B give rise to discrete cell populations within specific regions of the brain. Mitotic domains 1, 5, 9 and 20 give rise to brain neurons; mitotic domain B produced glial cells. Mitotic domains 5 and 9 produce the antennal and visual sensory systems, respectively, where each sensory system is composed of several disparate cell clusters. Time-lapse analysis of marked cells showed complex mitotic and migratory patterns for cells derived from these mitotic domains.
Jonathan Minden
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Advances in experimental medicine and biology     Volume:  628     ISSN:  0065-2598     ISO Abbreviation:  Adv. Exp. Med. Biol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-08-07     Completed Date:  2008-08-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0121103     Medline TA:  Adv Exp Med Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  57-68     Citation Subset:  IM    
Department of Biological Sciences and Science, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA. minden@cmuedu
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MeSH Terms
Brain / cytology,  embryology*,  metabolism
Cell Lineage
Drosophila / embryology*,  genetics
Embryo, Nonmammalian / cytology,  embryology*,  metabolism
Gene Expression Regulation, Developmental
Green Fluorescent Proteins / genetics,  metabolism
Microscopy, Fluorescence
Models, Biological
Morphogenesis / genetics
Reg. No./Substance:
147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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