| Dissecting timing variability in yeast meiosis. | |
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MedLine Citation:
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PMID: 17981121 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell-to-cell variability in the timing of cell-fate changes can be advantageous for a population of single-celled organisms growing in a fluctuating environment. We study timing variability during meiosis in Saccharomyces cerevisiae, initiated upon nutritional starvation. We use time-lapse fluorescence microscopy to measure the timing of meiotic events in single cells and find that the duration of meiosis is highly variable between cells. This variability is concentrated between the beginning of starvation and the onset of early meiosis genes. Cell-cycle variability and nutritional history have little effect on this timing variability. Rather, variation in the production rate of the meiotic master regulator Ime1 and its gradual increase over time govern this variability, and cell size effects are channeled through Ime1. These results tie phenotypic variability with expression dynamics of a transcriptional regulator and provide a general framework for the study of temporal developmental processes. |
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Authors:
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Iftach Nachman; Aviv Regev; Sharad Ramanathan |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell Volume: 131 ISSN: 0092-8674 ISO Abbreviation: Cell Publication Date: 2007 Nov |
Date Detail:
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Created Date: 2007-11-05 Completed Date: 2007-12-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0413066 Medline TA: Cell Country: United States |
Other Details:
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Languages: eng Pagination: 544-56 Citation Subset: IM |
Affiliation:
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FAS Center for System Biology, Harvard University, Cambridge, MA 02138, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle Proteins
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metabolism Cell Nucleus / metabolism DNA-Binding Proteins / metabolism Food Genes, Fungal Genes, Reporter Meiosis* Nuclear Proteins / metabolism Phenotype Saccharomyces cerevisiae / cytology*, genetics Saccharomyces cerevisiae Proteins / metabolism Signal Transduction Time Factors Transcription Factors / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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P50 GM068763/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/DMC1 protein, S cerevisiae; 0/DNA-Binding Proteins; 0/IME1 protein, S cerevisiae; 0/Nuclear Proteins; 0/Saccharomyces cerevisiae Proteins; 0/Transcription Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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