Document Detail


Dissecting regional variations in stress fiber mechanics in living cells with laser nanosurgery.
MedLine Citation:
PMID:  21044574     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ability of a cell to distribute contractile stresses across the extracellular matrix in a spatially heterogeneous fashion underlies many cellular behaviors, including motility and tissue assembly. Here we investigate the biophysical basis of this phenomenon by using femtosecond laser nanosurgery to measure the viscoelastic recoil and cell-shape contributions of contractile stress fibers (SFs) located in specific compartments of living cells. Upon photodisruption and recoil, myosin light chain kinase-dependent SFs located along the cell periphery display much lower effective elasticities and higher plateau retraction distances than Rho-associated kinase-dependent SFs located in the cell center, with severing of peripheral fibers uniquely triggering a dramatic contraction of the entire cell within minutes of fiber irradiation. Image correlation spectroscopy reveals that when one population of SFs is pharmacologically dissipated, actin density flows toward the other population. Furthermore, dissipation of peripheral fibers reduces the elasticity and increases the plateau retraction distance of central fibers, and severing central fibers under these conditions triggers cellular contraction. Together, these findings show that SFs regulated by different myosin activators exhibit different mechanical properties and cell shape contributions. They also suggest that some fibers can absorb components and assume mechanical roles of other fibers to stabilize cell shape.
Authors:
Kandice Tanner; Aaron Boudreau; Mina J Bissell; Sanjay Kumar
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biophysical journal     Volume:  99     ISSN:  1542-0086     ISO Abbreviation:  Biophys. J.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-03     Completed Date:  2011-01-28     Revised Date:  2014-09-22    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2775-83     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Biomechanical Phenomena
Biophysical Phenomena
Cell Compartmentation
Cell Line, Tumor
Cell Shape / physiology
Elasticity
Extracellular Matrix / physiology
Glioma / physiopathology
Humans
Laser Therapy
Myosin-Light-Chain Kinase / physiology
Nanotechnology
Stress Fibers / physiology*
Viscosity
rho-Associated Kinases / physiology
Grant Support
ID/Acronym/Agency:
1DP2OD004213/OD/NIH HHS; 1U54CA143836/CA/NCI NIH HHS; R01 CA057621/CA/NCI NIH HHS; R01CA057621/CA/NCI NIH HHS; R37 CA064786/CA/NCI NIH HHS; R37CA064786/CA/NCI NIH HHS; U01CA143233/CA/NCI NIH HHS; U54CA112970/CA/NCI NIH HHS; U54CA126552/CA/NCI NIH HHS; U54CA143836/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
EC 2.7.11.1/rho-Associated Kinases; EC 2.7.11.18/Myosin-Light-Chain Kinase
Comments/Corrections

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