Document Detail


Disruption of sucA, which encodes the E1 subunit of alpha-ketoglutarate dehydrogenase, affects the survival of Nitrosomonas europaea in stationary phase.
MedLine Citation:
PMID:  16352852     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although Nitrosomonas europaea lacks measurable alpha-ketoglutarate dehydrogenase activity, the recent completion of the genome sequence revealed the presence of the genes encoding the enzyme. A knockout mutation was created in the sucA gene encoding the E1 subunit. Compared to wild-type cells, the mutant strain showed an accelerated loss of ammonia monooxygenase and hydroxylamine oxidoreductase activities upon entering stationary phase. In addition, unlike wild-type cells, the mutant strain showed a marked lag in the ability to resume growth in response to pH adjustments in late stationary phase.
Authors:
Norman G Hommes; Elizabeth G Kurth; Luis A Sayavedra-Soto; Daniel J Arp
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  188     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2005-12-14     Completed Date:  2006-03-14     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  343-7     Citation Subset:  IM    
Affiliation:
Department of Botany and Plant Pathology, Oregon State University, 2082 Cordley, Corvallis, OR 97331-2902, USA.
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MeSH Terms
Descriptor/Qualifier:
Gene Expression Regulation, Bacterial*
Gene Silencing*
Hydrogen-Ion Concentration
Ketoglutarate Dehydrogenase Complex / genetics*,  metabolism
Mutation
Nitrosomonas europaea / enzymology*,  genetics,  growth & development*,  physiology
Oxygen Consumption
Polymerase Chain Reaction
Chemical
Reg. No./Substance:
EC 1.2.4.2/Ketoglutarate Dehydrogenase Complex
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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