| Disruption of lipid rafts causes apoptotic cell death in HaCaT keratinocytes. | |
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MedLine Citation:
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PMID: 15810884 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lipid rafts are cholesterol-enriched microdomains in plasma membranes. The functional activity of many membrane proteins, including death and growth factor receptors, depends on their insertion in lipid rafts. We have previously demonstrated the presence of lipid rafts in keratinocytes and shown that lipid rafts are involved in the control of keratinocyte proliferation and metabolic activity. In this work, we investigated the effect of lipid-raft disruption on HaCaT keratinocyte survival. Lipid rafts could be disrupted or rearranged with cholesterol-targeting detergents: methyl-beta-cyclodextrin and filipin III. Moreover, cholesterol oxidation by a specific oxidase or blocking of cholesterol synthesis by mevastatin had a similar effect on lipid rafts. All cholesterol-modifying substances caused cell death in a concentration-dependent manner. More detailed studies on the effects of cyclodextrin revealed apoptotic cell death at concentrations >or=0.5% (w/v). The molecular mechanism of apoptosis precipitated by raft disruption remains unknown but does not seem to be dependent of either membrane permeabilization or cell-cycle arrest imposed by cholesterol-modifying compounds. |
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Authors:
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Bo Bang; Robert Gniadecki; Barbara Gajkowska |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Experimental dermatology Volume: 14 ISSN: 0906-6705 ISO Abbreviation: Exp. Dermatol. Publication Date: 2005 Apr |
Date Detail:
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Created Date: 2005-04-06 Completed Date: 2005-07-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9301549 Medline TA: Exp Dermatol Country: Denmark |
Other Details:
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Languages: eng Pagination: 266-72 Citation Subset: IM |
Affiliation:
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Department of Dermatology, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark. bb22@bbh.hosp.dk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis* Cell Cycle Cell Line, Tumor Cell Membrane / metabolism Cell Proliferation Cholesterol / chemistry, metabolism Cholesterol Oxidase / metabolism Detergents / pharmacology Enzyme-Linked Immunosorbent Assay Filipin / chemistry Humans In Situ Nick-End Labeling Keratinocytes / cytology* Lipids / chemistry* Lovastatin / analogs & derivatives*, chemistry Membrane Microdomains / chemistry* Microscopy, Confocal Microscopy, Electron Microscopy, Fluorescence Time Factors beta-Cyclodextrins / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Detergents; 0/Lipids; 0/beta-Cyclodextrins; 0/methyl-beta-cyclodextrin; 480-49-9/Filipin; 57-88-5/Cholesterol; 73573-88-3/compactin; 75330-75-5/Lovastatin; EC 1.1.3.6/Cholesterol Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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