Document Detail

Disruption of calreticulin-mediated cellular adhesion signaling in the cadmium-induced omphalocele in the chick model.
MedLine Citation:
PMID:  19865819     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Administration of cadmium (Cd) causes omphalocele in the chick embryo. The earliest histological changes in the chick Cd model are the breakdown of adherens junctions (AJs). Calreticulin (CRT) plays a key role in Ca(2+) signaling and cell adhesion. Ca(2+) signaling in the Cd chick model is known to be altered. The calcium-dependent adhesion molecule, E-cadherin, and its associate, beta-catenin, are key components of AJs regulated by CRT. CRT knockouts display omphalocele. We hypothesized that CRT, E-cadherin and beta-catenin are downregulated during early embryogenesis in the Cd chick model. METHODS: After 60 h (H) incubation, chicks were harvested 1H, 4H, and 8H post treatment with saline or Cd and divided into controls and Cd. RT-PCR was performed to evaluate mRNA levels of CRT, E-cadherin and beta-catenin in the Cd chick model. RESULTS: The mRNA levels of CRT were significantly decreased in the Cd group at 1H compared to controls (p < 0.05). The mRNA levels of E-cadherin and beta-catenin were significantly decreased at 4H in the Cd group compared to controls (p < 0.05). There were no significant differences at 8H. CONCLUSION: Downregulation of CRT, E-cadherin and beta-catenin genes may cause omphalocele in the Cd chick model by disrupting CRT-mediated Ca(2+) signaling and AJs.
Takashi Doi; Prem Puri; John Bannigan; Jennifer Thompson
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Pediatric surgery international     Volume:  26     ISSN:  1437-9813     ISO Abbreviation:  Pediatr. Surg. Int.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-01-21     Completed Date:  2010-04-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8609169     Medline TA:  Pediatr Surg Int     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  91-5     Citation Subset:  IM    
The Children's Research Centre, Our Lady's Children's Hospital, Dublin 12, Ireland.
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MeSH Terms
Cadherins / biosynthesis,  genetics
Cadmium / toxicity
Calcium-Binding Protein, Vitamin D-Dependent / genetics*,  metabolism
Cell Adhesion / physiology*
Chick Embryo
Disease Models, Animal
Gene Expression Regulation, Developmental*
Hernia, Umbilical / chemically induced,  embryology,  metabolism*
Nerve Tissue Proteins
RNA, Messenger / biosynthesis,  genetics*
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction / physiology*
beta Catenin / biosynthesis,  genetics
Reg. No./Substance:
0/Cadherins; 0/Calcium-Binding Protein, Vitamin D-Dependent; 0/Nerve Tissue Proteins; 0/RNA, Messenger; 0/beta Catenin; 0/calretinin; 7440-43-9/Cadmium

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