Document Detail

Disruption of bovine oocytes and preimplantation embryos by urea and acidic pH.
MedLine Citation:
PMID:  12741544     Owner:  NLM     Status:  MEDLINE    
Feeding cattle diets high in degradable crude protein (CP) or in excess of requirements can reduce fertility and lower uterine pH. Objectives were to determine direct effects of urea and acidic pH during oocyte maturation and embryonic development. For experiment 1, oocytes were matured in medium containing 0, 5, 7.5, or 10 mM urea (0, 14, 21, or 28 mg/dl urea nitrogen, respectively). Cleavage rate was not reduced by any concentration of urea. However, the proportion of oocytes developing to the blastocyst stage at d 8 after insemination was reduced by 7.5 mM urea. In addition, the proportion of cleaved oocytes becoming blastocysts was decreased by 5 and 7.5 mM urea. For experiment 2, putative zygotes were collected -9 h after insemination and cultured in modified Potassium Simplex Optimized Medium (KSOM). Urea did not reduce the proportion of oocytes developing to the blastocyst stage, although 10 mM urea reduced cleavage rate slightly. For experiment 3, dimethadione (DMD), a weak nonmetabolizable acid, was used to decrease culture medium pH. Putative zygotes were cultured in modified KSOM containing 0, 10, 15, or 20 mM DMD for 8 d. DMD reduced cleavage rate at 15 and 20 mM and development to the blastocyst stage at all concentrations. Results support the idea that feeding diets rich in highly degradable CP compromises fertility through direct actions of urea on the oocyte and through diet-induced alterations in uterine pH.
O M Ocon; P J Hansen
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of dairy science     Volume:  86     ISSN:  0022-0302     ISO Abbreviation:  J. Dairy Sci.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-05-13     Completed Date:  2003-06-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985126R     Medline TA:  J Dairy Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1194-200     Citation Subset:  IM    
Department of Animal Sciences, University of Florida, Gainesville, 32611-0910, USA.
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MeSH Terms
Cleavage Stage, Ovum / drug effects
Culture Media
Culture Techniques
Dimethadione / pharmacology
Embryonic Development
Embryonic and Fetal Development / drug effects
Fertilization in Vitro / veterinary
Hydrogen-Ion Concentration
Oocytes / drug effects*
Urea / pharmacology*
Zygote / drug effects*
Reg. No./Substance:
0/Culture Media; 57-13-6/Urea; 695-53-4/Dimethadione; 7440-09-7/Potassium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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