| Disruption of the Aspergillus fumigatus argB gene using a novel in vitro transposon-based mutagenesis approach. | |
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MedLine Citation:
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PMID: 14727059 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We disrupted the Aspergillus fumigatus argB gene, encoding ornithine transcarbamylase, using a novel in vitro transposon-based mutagenesis approach. This approach utilizes a modified transposon containing the Neurospora crassa pyr4 gene, which is randomly inserted in vitro into a target sequence of interest. Clones in which the gene of interest has been disrupted are identified by PCR and used to transform a pyrG-deficient strain of A. fumigatus. Using this approach, we obtained arginine auxotrophs of A. fumigatus. Full characterization of the argB insertion was performed by Southern blot analysis. These strains can be supplemented by addition of arginine into the culture medium and can be fully rescued to arginine prototrophy by transformation with the intact A. fumigatus argB gene. |
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Authors:
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Jeries Jadoun; Yona Shadkchan; Nir Osherov |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2004-01-15 |
Journal Detail:
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Title: Current genetics Volume: 45 ISSN: 0172-8083 ISO Abbreviation: Curr. Genet. Publication Date: 2004 Apr |
Date Detail:
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Created Date: 2004-03-26 Completed Date: 2004-12-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8004904 Medline TA: Curr Genet Country: United States |
Other Details:
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Languages: eng Pagination: 235-41 Citation Subset: IM |
Affiliation:
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Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, 69978, Tel Aviv, Israel. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aspergillus fumigatus
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genetics*,
metabolism Blotting, Southern DNA Transposable Elements* Gene Deletion Genetic Complementation Test Genetic Vectors Mutagenesis* Ornithine Carbamoyltransferase / genetics*, metabolism Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/DNA Transposable Elements; EC 2.1.3.3/Ornithine Carbamoyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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