Document Detail


Disruption of the Aspergillus fumigatus argB gene using a novel in vitro transposon-based mutagenesis approach.
MedLine Citation:
PMID:  14727059     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We disrupted the Aspergillus fumigatus argB gene, encoding ornithine transcarbamylase, using a novel in vitro transposon-based mutagenesis approach. This approach utilizes a modified transposon containing the Neurospora crassa pyr4 gene, which is randomly inserted in vitro into a target sequence of interest. Clones in which the gene of interest has been disrupted are identified by PCR and used to transform a pyrG-deficient strain of A. fumigatus. Using this approach, we obtained arginine auxotrophs of A. fumigatus. Full characterization of the argB insertion was performed by Southern blot analysis. These strains can be supplemented by addition of arginine into the culture medium and can be fully rescued to arginine prototrophy by transformation with the intact A. fumigatus argB gene.
Authors:
Jeries Jadoun; Yona Shadkchan; Nir Osherov
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-01-15
Journal Detail:
Title:  Current genetics     Volume:  45     ISSN:  0172-8083     ISO Abbreviation:  Curr. Genet.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-03-26     Completed Date:  2004-12-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8004904     Medline TA:  Curr Genet     Country:  United States    
Other Details:
Languages:  eng     Pagination:  235-41     Citation Subset:  IM    
Affiliation:
Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, 69978, Tel Aviv, Israel.
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MeSH Terms
Descriptor/Qualifier:
Aspergillus fumigatus / genetics*,  metabolism
Blotting, Southern
DNA Transposable Elements*
Gene Deletion
Genetic Complementation Test
Genetic Vectors
Mutagenesis*
Ornithine Carbamoyltransferase / genetics*,  metabolism
Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/DNA Transposable Elements; EC 2.1.3.3/Ornithine Carbamoyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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