| Disposable luminol copolymer-based biosensor for uric acid in urine. | |
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MedLine Citation:
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PMID: 21839206 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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A new electrochemiluminescent (ECL) disposable biosensor for uric acid was manufactured by immobilization in a double-layer design of luminol as a copolymer with 3,3',5,5'-tetramethylbenzidine (TMB) and the enzyme uricase in chitosan on gold screen-printed cells. The good mechanical and improved electroluminescent characteristics of the new copolymer poly(luminol-TMB) make it possible to determine uric acid by measuring the growing ECL emission with the analyte concentration. The combination of enzymatic selectivity with ECL sensitivity results in a disposable analytical device with a linear range for uric acid from 1.5×10(-6) to 1.0×10(-4)M, a limit of detection of 4.4×10(-7)M and a precision of 13.1% (1.0×10(-5)M, n=10) as relative standard deviation. Satisfactory results were obtained for uric acid determination in 24h-urine samples compared to a reference procedure. This uric acid biosensor can be used as a low-cost alternative to conventional methods. |
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Authors:
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J Ballesta-Claver; I F Díaz Ortega; M C Valencia-Mirón; L F Capitán-Vallvey |
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Publication Detail:
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Type: Journal Article Date: 2011-07-05 |
Journal Detail:
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Title: Analytica chimica acta Volume: 702 ISSN: 1873-4324 ISO Abbreviation: Anal. Chim. Acta Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-08-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370534 Medline TA: Anal Chim Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 254-61 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier B.V. All rights reserved. |
Affiliation:
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ECsens, Department of Analytical Chemistry, Campus Fuentenueva, Faculty of Sciences, University of Granada, E-18071 Granada, Spain. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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