| Displacement of bilirubin from adult and newborn serum albumin by a drug and fatty acid. | |
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MedLine Citation:
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PMID: 2566458 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Competitive binding of bilirubin and 5-butyl-1-cyclohexylbarbituric acid (bucolome) to human serum albumin was investigated by equilibrium dialysis, with and without added bilirubin (0.5 mol/mol albumin). Further observations were made by peroxidase oxidation kinetics. Finally, cobinding of the two ligands was studied by spectrophotometry. Experiments were performed with defatted adult human serum albumin, with umbilical cord serum, and with adult albumin to which was bound 2 mol palmitate/mol albumin. Bucolome does not induce displacement of bilirubin from binding to defatted adult albumin when one molecule of either ligand is bound. The second molecule of bound bucolome displaces the first bilirubin molecule. With albumin in umbilical cord serum, the first bucolome does decrease binding of the first bilirubin to some extent although the two ligands can bind to the same albumin molecule. The presence of two molecules of bound palmitate together with one of bilirubin causes enhanced bilirubin displacement on binding of bucolome. These observations may be of consequence for practical testing of the bilirubin displacing effect of drugs. Experiments with neonatal albumin and with added fatty acid should be included for a complete study. |
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Authors:
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R Ivarsen; R Brodersen |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Developmental pharmacology and therapeutics Volume: 12 ISSN: 0379-8305 ISO Abbreviation: Dev Pharmacol Ther Publication Date: 1989 |
Date Detail:
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Created Date: 1989-07-03 Completed Date: 1989-07-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8003947 Medline TA: Dev Pharmacol Ther Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 19-29 Citation Subset: IM |
Affiliation:
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Institute of Medical Biochemistry, University of Aarhus, Denmark. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Age Factors Barbiturates / metabolism* Bilirubin / metabolism* Binding, Competitive Drug Interactions Fetal Blood / metabolism* Humans Infant, Newborn Palmitates / pharmacology* Palmitic Acids / pharmacology* Protein Binding Serum Albumin / metabolism* Spectrophotometry |
| Chemical | |
Reg. No./Substance:
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0/Barbiturates; 0/Palmitates; 0/Palmitic Acids; 0/Serum Albumin; 635-65-4/Bilirubin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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