Document Detail


Dispersed donor salivary gland cells are widely distributed in the recipient gland when infused up the ductal tree.
MedLine Citation:
PMID:  19572222     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The salivary glands often are severely and permanently damaged by therapeutic irradiation for cancer of the head and neck. The markedly reduced quantity and quality of saliva results in greatly increased susceptibility to dental caries and infection of the oral mucosa and alveolar bone. Recently, subcapsular injection of cultured mouse salivary gland cells has achieved a significant degree of regeneration in a previously irradiated mouse salivary gland; however, the recovery was limited to one lobule. We describe here a method for delivering donor rat salivary gland cells via the main duct that distributes several thousand cells throughout the recipient rat's salivary gland. The donated cells exhibited the cytodifferentiation of the structures in which they lodged, i.e., acini, granular convoluted tubules, and the several types of ducts. This method may facilitate the simultaneous functional recovery of almost all of the lobules of irradiated rat salivary glands.
Authors:
R S Redman; W D Ball; E Mezey; S Key
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biotechnic & histochemistry : official publication of the Biological Stain Commission     Volume:  84     ISSN:  1473-7760     ISO Abbreviation:  Biotech Histochem     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2010-05-27     Completed Date:  2010-06-25     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  9107378     Medline TA:  Biotech Histochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  253-60     Citation Subset:  IM    
Affiliation:
Oral Pathology Research Laboratory (151-I), Department of Veterans Affairs Medical Center, 50 Irving Street NW, Washington, DC 20422, USA. oralpath@erols.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques
Cell Transplantation / methods*
Cells, Cultured
Female
Male
Mouth Mucosa / physiopathology
Rats
Rats, Sprague-Dawley
Recovery of Function*
Regeneration*
Saliva
Salivary Gland Diseases / physiopathology*
Salivary Glands / cytology*,  radiation effects
Specific Pathogen-Free Organisms
Tissue Donors
Grant Support
ID/Acronym/Agency:
DE 14995/DE/NIDCR NIH HHS; R21 DE014995-02/DE/NIDCR NIH HHS
Comments/Corrections
Erratum In:
Biotech Histochem. 2010 Feb;85(1):87

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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