| The Dispersal State of Multi-walled Carbon Nanotubes Elicits Pro-Fibrogenic Cellular Responses that Correlate with Fibrogenesis Biomarkers and Fibrosis in the Murine Lung. | |
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MedLine Citation:
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PMID: 22047207 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We developed a dispersal method for multi-walled carbon nanotubes (MWCNTs) that allows quantitative assessment of dispersion on pro-fibrogenic responses in tissue culture cells as well as in mouse lung. Here we demonstrate that the dispersal of as-prepared (AP), purified (PD), and carboxylated (COOH) MWCNTs by bovine serum albumin (BSA) and dipalmitoylphosphatidylcholine (DPPC) influences TGF-β1, PDGF-AA and IL-1β production in vitro and in vivo. These biomarkers were chosen based on their synergy in promoting fibrogenesis and cellular communication in the epithelial-mesenchymal cell trophic unit in the lung. The effect of dispersal was most noticeable in AP- and PD-MWCNTs, which are more hydrophobic and unstable in aqueous buffers than hydrophilic COOH-MWCNTs. Well-dispersed AP- and PD-MWCNTs were readily taken up by BEAS-2B, THP-1 cells and alveolar macrophages (AM), and induced more prominent TGF-β1 and IL-1β production in vitro as well as TGF-β1, IL-1β and PDGF-AA production in vivo than non-dispersed tubes. Moreover, there was good agreement between the pro-fibrogenic responses in vitro and in vivo as well as the ability of dispersed tubes to generate granulomatous inflammation and fibrosis in airways. Tube dispersal also elicited more robust IL-1β production in THP-1 cells. While COOH-MWCNTs were poorly taken up in BEAS-2B and induced little TGF-β1 production, they were bio-processed by AM and induced less prominent collagen deposition at sites of non-granulomatous inflammation in the alveolar region. Taken together, these results indicate that the dispersal state of MWCNTs affects pro-fibrogenic cellular responses that correlate with the extent of pulmonary fibrosis and are of potential use to predict pulmonary toxicity. |
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Authors:
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Xiang Wang; Tian Xia; Susana Addo Ntim; Zhaoxia Ji; Sijie Lin; Huan Meng; Choong-Heui Chung; Saji George; Haiyuan Zhang; Meiying Wang; Ning Li; Yang Yang; Vincent Castranova; Somenath Mitra; James C Bonner; Andre E Nel |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-2 |
Journal Detail:
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Title: ACS nano Volume: - ISSN: 1936-086X ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-3 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101313589 Medline TA: ACS Nano Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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