Document Detail


Dispensability of p53 degradation for tumorigenicity and decreased serum requirement of human papillomavirus type 16 E6.
MedLine Citation:
PMID:  9537653     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Certain types of human papillomavirus (HPV), such as types 16 and 18, are etiological agents for carcinogenesis of the uterine cervix. These HPVs have two oncogenes, E6 and E7, that have transforming activities in established murine cells. Tumorigenicity and decreased serum requirement for cell growth are conferred by the E6 gene, whereas anchorage-independent growth is mainly governed by the E7 gene. To understand the mechanism of cellular transformation by the HPV16 E6 gene, we examined three mutant E6 proteins defective for p53 binding, p53 degradation, or transactivation of the adenovirus E2 promoter for the ability to induce tumorigenicity and decreased serum requirement. The results showed that tumorigenicity and decreased serum requirement were associated with the ability of E6 to bind to p53, although the subsequent degradation of p53 was not required for these functions.
Authors:
T Inoue; K Oka; H Yong-Il; K H Vousden; S Kyo; P Jing; A Hakura; M Yutsudo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular carcinogenesis     Volume:  21     ISSN:  0899-1987     ISO Abbreviation:  Mol. Carcinog.     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-04-16     Completed Date:  1998-04-16     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8811105     Medline TA:  Mol Carcinog     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  215-22     Citation Subset:  IM    
Affiliation:
Department of Tumor Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Transformation, Neoplastic*
Culture Media, Serum-Free
Gene Expression
Genes, Viral
Genetic Vectors
Humans
Mice
Mutation
Oncogene Proteins, Viral / biosynthesis,  genetics*
Papillomaviridae / genetics*
Promoter Regions, Genetic / physiology
Repressor Proteins*
Transcriptional Activation / physiology
Transfection
Transformation, Genetic
Tumor Suppressor Protein p53 / biosynthesis,  metabolism*
Chemical
Reg. No./Substance:
0/Culture Media, Serum-Free; 0/E6 protein, Human papillomavirus type 16; 0/Oncogene Proteins, Viral; 0/Repressor Proteins; 0/Tumor Suppressor Protein p53

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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