Document Detail


Disinfection of ocular cells and tissues by atmospheric-pressure cold plasma.
MedLine Citation:
PMID:  22432007     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Low temperature plasmas have been proposed in medicine as agents for tissue disinfection and have received increasing attention due to the frequency of bacterial resistance to antibiotics. This study explored whether atmospheric-pressure cold plasma (APCP) generated by a new portable device that ionizes a flow of helium gas can inactivate ocular pathogens without causing significant tissue damage.
METHODOLOGY/PRINCIPAL FINDINGS: We tested the APCP effects on cultured Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Candida albicans, Aspergillus fumigatus and Herpes simplex virus-1, ocular cells (conjunctival fibroblasts and keratocytes) and ex-vivo corneas. Exposure to APCP for 0.5 to 5 minutes significantly reduced microbial viability (colony-forming units) but not human cell viability (MTT assay, FACS and Tunel analysis) or the number of HSV-1 plaque-forming units. Increased levels of intracellular reactive oxygen species (ROS) in exposed microorganisms and cells were found using a FACS-activated 2',7'-dichlorofluorescein diacetate probe. Immunoassays demonstrated no induction of thymine dimers in cell cultures and corneal tissues. A transient increased expression of 8-OHdG, genes and proteins related to oxidative stress (OGG1, GPX, NFE2L2), was determined in ocular cells and corneas by HPLC, qRT-PCR and Western blot analysis.
CONCLUSIONS: A short application of APCP appears to be an efficient and rapid ocular disinfectant for bacteria and fungi without significant damage on ocular cells and tissues, although the treatment of conjunctival fibroblasts and keratocytes caused a time-restricted generation of intracellular ROS and oxidative stress-related responses.
Authors:
Paola Brun; Paola Brun; Maria Vono; Paola Venier; Elena Tarricone; Velika Deligianni; Emilio Martines; Matteo Zuin; Silvia Spagnolo; Roberto Cavazzana; Romilda Cardin; Ignazio Castagliuolo; Alvise La Gloria Valerio; Andrea Leonardi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-03-14
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-03-20     Completed Date:  2012-07-13     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e33245     Citation Subset:  IM    
Affiliation:
Histology Unit, Department of Biomedical Sciences, University of Padua, Padua, Italy. paola.brun@unipd.it
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MeSH Terms
Descriptor/Qualifier:
Acetylcysteine / pharmacology
Adult
Atmospheric Pressure*
Cell Cycle / drug effects
Cell Survival / drug effects
Conjunctiva / cytology
Corneal Keratocytes / cytology*,  metabolism
Deoxyguanosine / analogs & derivatives,  metabolism
Disinfection / methods*
Exocytosis / drug effects
Eye / cytology*,  drug effects*
Fibroblasts / cytology*,  drug effects
Humans
Microbial Viability / drug effects
Phosphatidylserines / metabolism
Plasma Gases / pharmacology*
Protein Biosynthesis / drug effects
Reactive Oxygen Species / metabolism
Respiratory Burst / drug effects
Time Factors
Transcription, Genetic / drug effects
Virus Inactivation / drug effects
Chemical
Reg. No./Substance:
0/8-hydroxy-2'-deoxyguanosine; 0/Phosphatidylserines; 0/Plasma Gases; 0/Reactive Oxygen Species; 616-91-1/Acetylcysteine; 961-07-9/Deoxyguanosine
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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