| Discriminatory power of RAPD, PCR-RFLP and southern blot analyses of ureCD or ureA gene probes on Helicobacter pylori isolates. | |
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MedLine Citation:
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PMID: 12132695 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The genetic diversity of 33 Nigerian Helicobacter pylori isolates were studied using RAPD, PCR-RFLP and Southern blot analysis of ureA or ureCD gene probes. RAPD was able to distinguish the following number of isolates using the primers 3880: 5'-AAGAGCCCGT-3' (28), 3881 :5'-AACGCGCAAC-3' (33) and OPH8 :5'-GAAACACCCC-3' (25). Southern blot analysis using the ureCD probe was also able to distinguish the 12 isolates tested into ten different patterns. The PCR-RFLP technique distinguished all 33 isolates into six types. In conclusion, considering typeability, discriminatory power, and convenience, RAPD with the 3881 primer was considered the most useful technique. |
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Authors:
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Stella Smith; Franck Cantet; Fabrice Angelini; Armelle Marais; Francis Mégraud; Ekkhehard Bayerdöffer; Stephan Miehlke |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Zeitschrift für Naturforschung. C, Journal of biosciences Volume: 57 ISSN: 0939-5075 ISO Abbreviation: Z. Naturforsch., C, J. Biosci. Publication Date: 2002 May-Jun |
Date Detail:
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Created Date: 2002-07-22 Completed Date: 2002-09-20 Revised Date: 2009-11-04 |
Medline Journal Info:
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Nlm Unique ID: 8912155 Medline TA: Z Naturforsch C Country: Germany |
Other Details:
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Languages: eng Pagination: 516-21 Citation Subset: IM |
Affiliation:
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Molecular Biology and Biotechnology Division, Nigerian Institute of Medical Research, Yaba, Lagos. stellaismith@yahoo.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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genetics* DNA Primers Gastritis / microbiology Helicobacter Infections / microbiology Helicobacter pylori / genetics*, isolation & purification Humans Nigeria Phosphoglucomutase / genetics* Polymerase Chain Reaction / methods Polymorphism, Restriction Fragment Length Random Amplified Polymorphic DNA Technique / methods Sensitivity and Specificity Ulcer / microbiology Urease / genetics* |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/DNA Primers; 0/UreD protein, Bacteria; EC 3.5.1.5/Urease; EC 5.4.2.-/phosphoglucosamine mutase; EC 5.4.2.2/Phosphoglucomutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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