Document Detail

Discriminatory power of RAPD, PCR-RFLP and southern blot analyses of ureCD or ureA gene probes on Helicobacter pylori isolates.
MedLine Citation:
PMID:  12132695     Owner:  NLM     Status:  MEDLINE    
The genetic diversity of 33 Nigerian Helicobacter pylori isolates were studied using RAPD, PCR-RFLP and Southern blot analysis of ureA or ureCD gene probes. RAPD was able to distinguish the following number of isolates using the primers 3880: 5'-AAGAGCCCGT-3' (28), 3881 :5'-AACGCGCAAC-3' (33) and OPH8 :5'-GAAACACCCC-3' (25). Southern blot analysis using the ureCD probe was also able to distinguish the 12 isolates tested into ten different patterns. The PCR-RFLP technique distinguished all 33 isolates into six types. In conclusion, considering typeability, discriminatory power, and convenience, RAPD with the 3881 primer was considered the most useful technique.
Stella Smith; Franck Cantet; Fabrice Angelini; Armelle Marais; Francis Mégraud; Ekkhehard Bayerdöffer; Stephan Miehlke
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zeitschrift für Naturforschung. C, Journal of biosciences     Volume:  57     ISSN:  0939-5075     ISO Abbreviation:  Z. Naturforsch., C, J. Biosci.     Publication Date:    2002 May-Jun
Date Detail:
Created Date:  2002-07-22     Completed Date:  2002-09-20     Revised Date:  2009-11-04    
Medline Journal Info:
Nlm Unique ID:  8912155     Medline TA:  Z Naturforsch C     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  516-21     Citation Subset:  IM    
Molecular Biology and Biotechnology Division, Nigerian Institute of Medical Research, Yaba, Lagos.
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MeSH Terms
Bacterial Proteins / genetics*
DNA Primers
Gastritis / microbiology
Helicobacter Infections / microbiology
Helicobacter pylori / genetics*,  isolation & purification
Phosphoglucomutase / genetics*
Polymerase Chain Reaction / methods
Polymorphism, Restriction Fragment Length
Random Amplified Polymorphic DNA Technique / methods
Sensitivity and Specificity
Ulcer / microbiology
Urease / genetics*
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA Primers; 0/UreD protein, Bacteria; EC; EC 5.4.2.-/phosphoglucosamine mutase; EC

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