Document Detail

Discriminative EPR detection of NO and HNO by encapsulated nitronyl nitroxides.
MedLine Citation:
PMID:  23136998     Owner:  NLM     Status:  MEDLINE    
Nitric oxide, •NO, is one of the most important molecules in the biochemistry of living organisms. By contrast, nitroxyl, NO-, one-electron reduced analog of •NO which exists at physiological conditions in its protonated form, HNO, has been relatively overlooked. Recent data show that HNO might be produced endogenously and display unique biological effects. However, there is a lack of specific and quantitative methods of detection of endogenous HNO production. Here we present a new method for discriminative •NO and HNO detection by nitronyl nitroxides (NNs) using electron paramagnetic resonance (EPR). It was found that NNs react with •NO and HNO with similar rate constants of about 10(4) M(-1) s(-1) but yield different products: imino nitroxides and the hydroxylamine of imino nitroxides, correspondingly. An EPR approach for discriminative •NO and HNO detection using liposome-encapsulated NNs was developed. The membrane barrier of liposomes protects NNs against reduction in biological systems while is permeable to both analytes, •NO and HNO. The sensitivity of this approach for the detection of the rates of •NO/HNO generation is about 1 nM/s. The application of encapsulated NNs for real-time discriminative •NO/HNO detection might become a valuable tool in nitric oxide-related studies.
Andrey A Bobko; Alexander Ivanov; Valery V Khramtsov
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-11-28
Journal Detail:
Title:  Free radical research     Volume:  47     ISSN:  1029-2470     ISO Abbreviation:  Free Radic. Res.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-09     Completed Date:  2013-06-25     Revised Date:  2014-04-10    
Medline Journal Info:
Nlm Unique ID:  9423872     Medline TA:  Free Radic Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  74-81     Citation Subset:  IM    
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MeSH Terms
Electron Spin Resonance Spectroscopy*
Hydroxylamine / analysis,  chemistry
Nitric Oxide / analysis*,  chemistry
Nitrogen Oxides / analysis*,  chemistry
Grant Support
Reg. No./Substance:
0/Liposomes; 0/Nitrogen Oxides; 14332-28-6/nitroxyl; 2FP81O2L9Z/Hydroxylamine; 31C4KY9ESH/Nitric Oxide

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