| Discrimination of viable and non-viable cells using propidium iodide in two color immunofluorescence. | |
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MedLine Citation:
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PMID: 3113897 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The relative ease with which a flow cytometer can perform simultaneous two color immunofluorescence to examine subpopulations of lymphoid cells has been well documented. Thus, flow cytometers equipped with only a single argon laser can be used to delineate various cell types by exciting both fluorescein- and phycoerythrin-conjugated antibodies to cell surface antigens. One problem that remains, however, is the artifactual staining of dead cells and clumps, which cannot be distinguished from viable cells on the basis of cell surface staining characteristics. We describe a method for simultaneous two color analysis or sorting of viable leukocytes which requires only a single laser. The method utilizes propidium iodide, which stains dead cells and thereby excludes such cells from the analysis. Using this method, as many as four viable cell types have been simultaneously analyzed in a single sample. |
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Authors:
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D T Sasaki; S E Dumas; E G Engleman |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cytometry Volume: 8 ISSN: 0196-4763 ISO Abbreviation: Cytometry Publication Date: 1987 Jul |
Date Detail:
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Created Date: 1987-10-22 Completed Date: 1987-10-22 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8102328 Medline TA: Cytometry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 413-20 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line Cell Survival Flow Cytometry / methods* Fluorescein-5-isothiocyanate Fluoresceins / diagnostic use Fluorescent Antibody Technique* Humans Lasers Light Phenanthridines / diagnostic use* Phycoerythrin / diagnostic use Propidium / diagnostic use* Scattering, Radiation T-Lymphocytes / cytology* Thiocyanates / diagnostic use |
| Grant Support | |
ID/Acronym/Agency:
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HL33811/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fluoresceins; 0/Phenanthridines; 0/Thiocyanates; 11016-17-4/Phycoerythrin; 3326-32-7/Fluorescein-5-isothiocyanate; 36015-30-2/Propidium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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