Document Detail


Discrimination of neolacto-series gangliosides with alpha2-3- and alpha2-6-linked N-acetylneuraminic acid by nanoelectrospray ionization low-energy collision-induced dissociation tandem quadrupole TOF MS.
MedLine Citation:
PMID:  14588011     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A combined strategy of thin-layer chromatography immunostaining and negative ion nanoelectrospray low-energy CID mass spectrometry was established for the differentiation of isomeric alpha2-3 and alpha2-6 sialylated neolacto-series monosialogangliosides from human granulocytes. The gangliosides investigated differed in the ceramide moiety by substitution with C16:0 or C24:1 fatty acid and in their oligosaccharide chains due to nLc4 and nLc6 core structures. With respect to the type of sialylation, the homogeneity of the HPLC-purified ganglioside fractions was verified by use of specific anti-Neu5Acalpha2-3Galbeta1-4GlcNAc-R and anti-Neu5Acalpha2-6Galbeta1-4GlcNAc-R antibodies. A clear-cut series of fragment ions for both types of isomeric gangliosides, carrying alpha2-3- and alpha2-6-linked neuraminic acid, respectively, was obtained by low-energy CID. Additionally, a characteristic ring cleavage was detected exclusively in all species with Neu5Acalpha2-6Galbeta1-4GlcNAc terminus, regardless of ceramide fatty acid and oligosaccharide chain lengths. The diagnostic (0,2)X(4/6) ions, generated by ring cleavage of an alpha2-6-linked neuraminic acid are accompanied by a simultaneous decrease of the corresponding Y(4)/Y(6) ions. These results suggest the unequivocal discrimination of individual alpha2-3- and alpha2-6-sialylated neolacto-series monosialogangliosides by distinct fragmentation patterns in low-energy CID tandem MS.
Authors:
Iris Meisen; Jasna Peter-Katalinić; Johannes Müthing
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical chemistry     Volume:  75     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-10-31     Completed Date:  2004-06-08     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5719-25     Citation Subset:  IM    
Affiliation:
Institute for Medical Physics and Biophysics, University of Münster, Robert-Koch-Strasse 31, D-48149 Münster, Germany.
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MeSH Terms
Descriptor/Qualifier:
Carbohydrate Conformation
Carbohydrate Sequence
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Disaccharides / analysis*,  chemistry,  immunology
Gangliosides / analysis*,  chemistry,  immunology
Granulocytes / chemistry
Humans
Immunoenzyme Techniques
Ions / chemistry
Isomerism
Molecular Sequence Data
Molecular Structure
Molecular Weight
Oligosaccharides / chemistry
Spectrometry, Mass, Electrospray Ionization* / methods*
Chemical
Reg. No./Substance:
0/Disaccharides; 0/Gangliosides; 0/Ions; 0/Oligosaccharides; 0/sialogangliosides; 35259-23-5/N-acetylneuraminyl-(2-6)-galactose; 83563-61-5/N-acetylneuraminyl-(2-3)-galactose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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