Document Detail

Discrimination between benign, primary and secondary malignancies in lymph nodes from the head and neck utilising Raman spectroscopy and multivariate analysis.
MedLine Citation:
PMID:  23295372     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The potential use of Raman spectroscopy (RS) for the detection of malignancy within lymph nodes of the head and neck was evaluated. RS measures the presence of biomolecules by the inelastic scattering of light within cells and tissues. This can be performed in vivo in real-time.
METHODS: 103 lymph nodes were collected from 23 patients undergoing surgery for suspicious lymph nodes. Five pathologies, defined by consensus histopathology, were collected including reactive nodes (benign), Hodgkin's and non-Hodgkin's lymphomas, metastases from both squamous cell carcinomas and adenocarcinomas. Raman spectra were measured with 830 nm excitation from numerous positions on each biopsy. Spectral diagnostic models were constructed using principal component analysis followed by linear discriminant analysis (PCA-LDA), and by partial least squares discriminant analysis (PLS-DA) for comparison. Two-group models were constructed to distinguish between reactive and malignant nodes, and three-group models to distinguish between the benign, primary and secondary conditions.
RESULTS: Results were validated using a repeated subsampling procedure. Sensitivities and specificities of 90% and 86% were obtained using PCA-LDA, and 89% and 88% using PLS-DA, for the two-group models. Both PCA-LDA and PLS-DA models were also found to be very successful at discriminating between pathologies in the three-group models achieving sensitivities and specificities of over 78% and 89% for PCA-LDA, and over 81% and 89% for PLS-DA for all three pathology groups.
CONCLUSION: Raman spectroscopy and chemometric techniques can be successfully utilised in combination for discriminating between different cancerous conditions of lymph nodes from the head and neck.
Gavin Rhys Lloyd; Linda E Orr; Jonathan Christie-Brown; Keith McCarthy; Simon Rose; Michael Thomas; Nicholas Stone
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Analyst     Volume:  138     ISSN:  1364-5528     ISO Abbreviation:  Analyst     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-06-18     Completed Date:  2013-11-19     Revised Date:  2014-03-14    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  England    
Other Details:
Languages:  eng     Pagination:  3900-8     Citation Subset:  IM    
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MeSH Terms
Adenocarcinoma / secondary*
Carcinoma, Squamous Cell / secondary*
Discriminant Analysis
Head and Neck Neoplasms / secondary*
Hodgkin Disease / pathology*
Least-Squares Analysis
Lymphatic Metastasis
Lymphoma, Non-Hodgkin / pathology*
Multivariate Analysis
Neoplasms, Second Primary / pathology*
Principal Component Analysis
Sensitivity and Specificity
Spectrum Analysis, Raman*
Grant Support
CSA/03/07/017//Department of Health

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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