| Discrete electrophoretic products of mitochondrial protein synthesis in the Chinese hamster ovary cell line. | |
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MedLine Citation:
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PMID: 912598 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mitochondrial proteins labelled with [35S]methionine for 1 h in whole Chinese hamster ovary (CHO) cells in the presence of cycloheximide or emetine, known inhibitors of cytosolic protein synthesis, have been enumerated and characterized by their electrophoretic mobility in sodium dodecyl sulfate slab gel electrophoresis. Ten distinct electrophoretic bands were observed. The components were relatively stable during a 2 h postlabelling period. The same 10 bands were also seen with the CHO cell line tsH1, labelled at 40 degrees C, a temperature at which cytosolic but not mitochondrial protein synthesis is inhibited in this cell line, and with isolated mitochondria labelled in the presence of cycloheximide. An 11th band was present when [3H]leucine but not [35S]methionine was used for labelling. The width of the major band suggested that it consists of two components making a total of at least 12 proteins synthesized in mitochondria. The molecular weights of these mitochondrial proteins ranged from 5000 to 50 000 and there was a sixfold difference in the relative molar amounts synthesized in a 1-h period in the presence of [3H]leucine or [35S]methionine. No differences in number or electrophoretic mobility of the mitochondrially synthesized proteins were found among the seven CHO cell lines examined. These results suggest the stability of the mitochondrial genome in the CHO cell line. |
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Authors:
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R W Yatscoff; K B Freeman |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Canadian journal of biochemistry Volume: 55 ISSN: 0008-4018 ISO Abbreviation: Can. J. Biochem. Publication Date: 1977 Oct |
Date Detail:
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Created Date: 1977-12-29 Completed Date: 1977-12-29 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0421034 Medline TA: Can J Biochem Country: CANADA |
Other Details:
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Languages: eng Pagination: 1064-74 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line Electrophoresis, Polyacrylamide Gel Genotype Leucine / metabolism Methionine / metabolism Mitochondria / metabolism* Molecular Weight Protein Biosynthesis* |
| Chemical | |
Reg. No./Substance:
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61-90-5/Leucine; 63-68-3/Methionine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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