Document Detail


Discrete analysis of serum uric acid with immobilized uricase and peroxidase.
MedLine Citation:
PMID:  10392568     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Commercially available uricase and peroxidase have been immobilized onto alkylamine glass and arylamine glass beads respectively. A discrete method has been developed to determine uric acid in serum using immobilized uricase and peroxidase. The method is based on generation of H2O2 from serum uric acid by immobilized uricase and its measurement by a colour reaction catalyzed by immobilized peroxidase. The minimum detection limit of the method was 8 microg/0.1 ml sample. The mean analytical recovery of added uric acid in serum was 87.5%. The within and between assay coefficient of variation (C.V.) were <6.58% and <10.77% respectively. The serum uric acid in apparently healthy adults and persons suffering from different disease was found to be 25-55 microg/ml, 32+/-2.25 (range, mean+/-S.D.) and 55-200 microg/ml; 52+/-6.4 (range, mean+/-S.D.) respectively by our method. A good correlation (r = 0.8170) was obtained between the serum urate values by this method and with those obtained by commercial Enzo-kit method.
Authors:
A K Bhargava; H Lal; C S Pundir
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biochemical and biophysical methods     Volume:  39     ISSN:  0165-022X     ISO Abbreviation:  J. Biochem. Biophys. Methods     Publication Date:  1999 May 
Date Detail:
Created Date:  1999-08-11     Completed Date:  1999-08-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7907378     Medline TA:  J Biochem Biophys Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  125-36     Citation Subset:  IM    
Affiliation:
Department of Bio-Sciences, Maharshi Dayanand University, Rohtak, India.
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MeSH Terms
Descriptor/Qualifier:
Colorimetry / methods*
Enzyme Stability
Enzymes, Immobilized / metabolism*
Horseradish Peroxidase / metabolism*
Humans
Reference Values
Urate Oxidase / metabolism*
Uric Acid / blood*
Chemical
Reg. No./Substance:
0/Enzymes, Immobilized; 69-93-2/Uric Acid; EC 1.11.1.-/Horseradish Peroxidase; EC 1.7.3.3/Urate Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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