Document Detail


Discrepancies in assignment of subtype/recombinant forms by genotyping programs for HIV type 1 drug resistance testing may falsely predict superinfection.
MedLine Citation:
PMID:  18593348     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
With the growing diversity of the HIV pandemic, routine genotyping is an important tool for monitoring viral subtype as well as drug resistance. In this regard, numerous subtyping tools and drug resistance algorithms are available online. However, there are discrepancies in the use of these online tools in the designation of HIV-1 subtypes or recombinant forms that may have an impact on drug susceptibility profiles. Indeed, inconsistencies in some of these tools may lead to a false designation of dual infection and/or superinfection. In this case study, we evaluated the sequence diversity of an infection that was referred to us as a potential case of superinfection as a result of variations in designation of subtype. We evaluated sequences using five different online tools and finally determined by phylogenetic analysis that the sequence was a unique A1/C intersubtype recombinant at baseline and not a case of superinfection.
Authors:
Michel Ntemgwa; M John Gill; Bluma G Brenner; Daniela Moisi; Mark A Wainberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  AIDS research and human retroviruses     Volume:  24     ISSN:  1931-8405     ISO Abbreviation:  AIDS Res. Hum. Retroviruses     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-08-01     Completed Date:  2008-10-14     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8709376     Medline TA:  AIDS Res Hum Retroviruses     Country:  United States    
Other Details:
Languages:  eng     Pagination:  995-1002     Citation Subset:  IM; X    
Affiliation:
McGill University AIDS Centre, Lady Davis Institute-Jewish General Hospital, Montreal, Quebec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Anti-HIV Agents / pharmacology*
Drug Resistance, Viral
False Positive Reactions
Genetic Variation
HIV Infections / virology*
HIV-1* / classification,  drug effects,  genetics
Humans
Male
Sequence Analysis*
Superinfection / diagnosis
Chemical
Reg. No./Substance:
0/Anti-HIV Agents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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