| Discovery of short, 3'-cholesterol-modified DNA duplexes with unique antitumor cell activity. | |
| | |
MedLine Citation:
|
PMID: 7954399 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A new class of modified oligodeoxynucleotides with unique, selective cytotoxic properties has been discovered. Self-complementary, 3'-cholesterol-modified oligodeoxynucleotides caused morphology changes and death in certain cancer cell lines, whereas other cell lines were unaffected. Susceptible cells were killed in a dose-dependent manner at submicromolar concentrations. Optimum potency was exhibited by phosphodiester duplexes approximately 10 base pairs in length, and base composition was important only in the context of duplex stability. Phosphorothioate analogues were less potent. Although the molecular mechanism of action of these unique compounds is not yet known, they offer potential applications in cancer therapy and in studies of cell death. In addition, the path toward elucidation of the structure-based biological activity of these oligonucleotides should be especially instructive for researchers studying sequence-specific effects. |
| | |
Authors:
|
J H Zhou; B S Pai; M W Reed; H B Gamper; E Lukhtanov; M Podyminogin; R B Meyer; Y C Cheng |
Related Documents
:
|
19579799 - Anti-tumor studies with extracts of calotropis procera (ait.) r.br. root employing hep2... 3015389 - Effect of o6-alkylguanine pretreatment on the sensitivity of human colon tumor cells to... 16824549 - Selective cytotoxicity of withaphysalins in myeloid leukemia cell lines versus peripher... 19875299 - Synthesis and biological evaluation of 3',4',5'-trimethoxychalcone analogues as inhibit... 20102369 - Antiproliferative activity of purine nucleoside phosphorylase multisubstrate analogue i... 22382319 - A novel and facile approach to imaging nanoparticles transport across transwell filter ... 8989889 - Protein farnesyltransferase in plants: molecular characterization and involvement in ce... 2143509 - Adenovirus transformed monkey cell lines permissive to enteric adenovirus type 40. 11050009 - Regulation of hemoglobin synthesis and proliferation of differentiating erythroid cells... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Cancer research Volume: 54 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1994 Nov |
Date Detail:
|
Created Date: 1994-12-02 Completed Date: 1994-12-02 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 5783-7 Citation Subset: IM |
Affiliation:
|
Department of Pharmacology, School of Medicine, Yale University, Sterling Hall of Medicine, New Haven, Connecticut 06510. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Base Sequence Carcinoma, Hepatocellular / pathology Cholesterol / chemistry Colonic Neoplasms / pathology Dose-Response Relationship, Drug Drug Design Drug Screening Assays, Antitumor Humans Mice Molecular Sequence Data Oligonucleotides, Antisense / chemistry, pharmacology* Oncogenes / genetics Structure-Activity Relationship Tumor Cells, Cultured / drug effects, pathology Tumor Stem Cell Assay |
| Grant Support | |
ID/Acronym/Agency:
|
AI29277-03/AI/NIAID NIH HHS; CA09085-18/CA/NCI NIH HHS; CA44358/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Oligonucleotides, Antisense; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Relationship between the structure of taxol and other taxanes on induction of tumor necrosis factor-...
Next Document: Detection of the M(r) 190,000 multidrug resistance protein, MRP, with monoclonal antibodies.