Document Detail


Discovery of an 11 (R)- and 12(S)-lipoxygenase activity in ovaries of the mussel Mytilus edulis.
MedLine Citation:
PMID:  11132179     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Eicosanoid biosynthesis was investigated in mussel gonads by incubation of tissue homogenates with radiolabeled arachidonic acid and analysis of the products by radio-high-performance liquid chromatography. No radiolabeled metabolites were formed in homogenates of testes, but two major metabolites were synthesized by ovarian preparations. The radiolabeled metabolites were analyzed by mass spectrometry and chiral chromatography and identified as 11 (R)-hydroxy-5,8,1 2,14-eicosatetraenoic acid and 12(S)-hydroxy-5,8,10,14-eicosatetraenoic acid. In addition, four other nonlabeled metabolites, formed from endogenous substrates, were detected in ovarian extracts. Their structures, determined by mass spectrometric analysis, were the corresponding 11- and 12-hydroxy analogs formed from eicosapentaenoic acid (11-HEPE and 12-HEPE) and 9-hydroxy-6,10,12,15-octadecatetraenoic acid (9-HOTE) and 13-hydroxy-6,9,11,15-octadecatetraenoic acid formecl from stearidonic acid. The biosynthesis of the 11 - and 12-hydroxy products was calcium dependent, localized to the 100,000 x g supernatant cell fraction, and was inhibited by nordihydroguaiaretic acid, but not inhibited by the prostaglandin synthase inhibitors aspirin and indomethacin, or the monoxygenase inhibitor proadifen. Together these data suggested that both the 11 (R)- and 12(S)-hydroxy products were formed from lipoxygenase-type enzymes. Incubation of homogenates of immature ovaries with eicosapentaenoic acid revealed the major product to be I2-HEPE, whereas in mature ovaries mainly 11-HEPE was formed. Extraction of spawned eggs with methanol revealed that predominantly 11-HEPE and 9-HOTE were formed from endogenous substrates. This study shows that female gonads of the mussel express an 11(R)- and 12(S)-lipoxygenase activity whose expression is dependent on differentiation of the ovary.
Authors:
G Coffa; E M Hill
Related Documents :
10318799 - Ahnak, a protein that binds and activates phospholipase c-gamma1 in the presence of ara...
190649 - Elevation of prostaglandin and cyclic amp levels by arachidonic acid in primary epithel...
2838699 - In vitro study of frog adrenal function--ix. evidence against the involvement of lipoxy...
6434639 - Lipoxygenation of arachidonic acid by subcellular preparations from murine keratinocytes.
2866439 - Chronic arachidonic acid eicosanoid imbalance: a common feature in coronary artery dise...
1902839 - Gas chromatographic-mass spectrometric characterisation of some novel hydroxyeicosatetr...
24731109 - Negative electrospray ionization via deprotonation - predicting the ionization efficiency.
11477599 - Localization and quantification of 5-hydroxytryptophan and serotonin in the central ner...
18624429 - Reducing acrylamide precursors in raw materials derived from wheat and potato.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Lipids     Volume:  35     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2000-12-22     Completed Date:  2001-02-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1195-204     Citation Subset:  IM    
Affiliation:
School of Chemistry, Physics and Environmental Science, University of Sussex Falmer, Brighton, United Kingdom.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Arachidonate 12-Lipoxygenase / metabolism*
Arachidonate Lipoxygenases / metabolism*
Bivalvia
Calcium / metabolism
Chromatography, High Pressure Liquid
Female
Gas Chromatography-Mass Spectrometry
Male
Ovary / enzymology*
Testis / enzymology
Chemical
Reg. No./Substance:
7440-70-2/Calcium; EC 1.13.11.-/11-lipoxygenase; EC 1.13.11.-/Arachidonate Lipoxygenases; EC 1.13.11.31/Arachidonate 12-Lipoxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Fatty acid chain length and degree of unsaturation are inversely associated with serum triglycerides...
Next Document:  Evidence for the mitochondrial biosynthesis of 3R-hydroxy-5Z,8Z,11Z,1 4Z-eicosatetraenoic acid in th...