Document Detail


Discontinuous RNA and protein synthesis and accumulation during cell cycle of Ehrlich ascites tumour cells.
MedLine Citation:
PMID:  4029278     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cell cycle-related synthesis rate and accumulation of RNA and protein were studied in in vivo growing Ehrlich ascites tumour cells. After pulse-labelling in vivo, cell fractions from various parts of the cell cycle were obtained by means of elutriator centrifuging. The RNA synthesis rate increased three times during G1 and remained unchanged throughout the remaining part of the cell cycle. The content of RNA increased discontinuously during G1/early S phase and late S phase/G2, by a factor of about 3. The protein synthesis rate increased by a factor of 2.5 and the protein content doubled, both parameters discontinuously as found for the RNA content. On the other hand, the cellular volume increased continuously throughout the cell cycle and thus no close correlation between cellular volume and protein content was indicated by our results. A possible variation in the degradation rate of RNA during cell cycle is also discussed. The close relationship between RNA content and protein synthesis during cell cycle indicates regulation of protein synthesis at the transcription level.
Authors:
S Skog; B Tribukait
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  159     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1985 Aug 
Date Detail:
Created Date:  1985-10-09     Completed Date:  1985-10-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  510-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoradiography
Carcinoma, Ehrlich Tumor / metabolism*
Cell Cycle
Centrifugation
Mice
Neoplasm Proteins / metabolism*
RNA, Neoplasm / biosynthesis
Chemical
Reg. No./Substance:
0/Neoplasm Proteins; 0/RNA, Neoplasm

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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