| Discoidin domain receptor 1 expression in activated T cells is regulated by the ERK MAP kinase signaling pathway. | |
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MedLine Citation:
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PMID: 21815193 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The expression and function of discoidin domain receptor 1 (DDR1) in T cells are still poorly explored. We have recently shown that activation of primary human T cells via their T cell receptor leads to increased expression of DDR1, which promoted their migration in three-dimensional collagen. In the present study, we provide evidence that activated T cells bind collagen through DDR1. We found that the DDR1:Fc blocking molecule significantly reduced the ability of activated T cells to bind soluble biotinylated collagen. However, DDR1:Fc had no impact on the adhesion of activated T cells to collagen and overexpression of DDR1 in Jurkat T cells did not enhance their adhesion. Together, our results indicate that DDR1 can promote T cell migration without enhancing adhesion to collagen, suggesting that it can contribute to the previously described amoeboid movement of activated T cells in collagen matrices. Our results also show that CD28, in contrast to IL-2 expression, did not costimulate the expression of DDR1 in primary human T cells. Using specific inhibitors, we demonstrated that TCR-induced expression of DDR1 in T cells is regulated by the Ras/Raf/ERK MAP Kinase and PKC pathways but not by calcium/calcineurin signaling pathway or the JNK and P38 MAP Kinases. Thus, our study provides additional insights into the physiology of DDR1 in T cells and may therefore further our understanding of the regulatory mechanisms of T cell migration. |
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Authors:
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Nizar Chetoui; Mohammed-Amine El Azreq; Marc Boisvert; Marie-Ève Bergeron; Fawzi Aoudjit |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cellular biochemistry Volume: 112 ISSN: 1097-4644 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-10-25 Completed Date: 2012-04-06 Revised Date: 2012-06-01 |
Medline Journal Info:
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Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 3666-74 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Wiley Periodicals, Inc. |
Affiliation:
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Centre de Recherche en Rhumatologie-Immunologie, Centre Hospitalier Universitaire de Québec, Pavillon CHUL, and Faculté de Médecine, Université Laval, 2705 Blvd Laurier, Local T1-49, Québec, QC, Canada G1V 4G2. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Calcium Signaling Humans Jurkat Cells Lymphocyte Activation MAP Kinase Signaling System* Polymerase Chain Reaction Receptor Protein-Tyrosine Kinases / metabolism* Receptors, Mitogen / metabolism* T-Lymphocytes / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Mitogen; EC 2.7.10.1/Receptor Protein-Tyrosine Kinases; EC 2.7.10.1/discoidin receptor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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