Document Detail

Disappearance of vimentin in Sertoli cells: a mono(2-ethylhexyl) phthalate effect.
MedLine Citation:
PMID:  17661219     Owner:  NLM     Status:  MEDLINE    
The effects of mono(2-ethylhexyl) phthalate (MEHP) on 21-day-old C57Bl/6N mice and their Sertoli cell cultures were studied. Mice were given a single dose of 800 mg/kg MEHP by oral gavage and sacrificed 24 h later. At the same time, testes were harvested from another batch of mice for Sertoli cell cultures. Cultures were subsequently exposed to 0, 1, and 100 nmol/ml MEHP for 0, 3, 6, 12, and 24 h. An antivimentin antibody was used to detect intermediate filament changes in Sertoli cells. Meanwhile, detection of preapoptotic signals and presence of apoptotic cells were done using annexin V-FITC (fluorescein isothiocyanate) and TUNEL (deoxynucleotidyltransferase-mediated dUTP nick end labeling) analyses, respectively. In vivo results showed a correlation between the increase in TUNEL-positive cells and the vimentin disruption in treated mice. Toluidine blue staining of the Sertoli cell cultures showed the increased number and size of vacuoles in Sertoli cell cytoplasm. Vimentin immunohistochemistry showed gradual disappearance of vimentin in Sertoli cell cultures as time and dose increased. Some Sertoli cells were found to be annexin V-FITC positive, but no TUNEL-positive cells were found. Taken together, these results show that the appearance of vacuoles and the vimentin disappearance caused by MEHP in the Sertoli cells are related with each other and can be observed in relation to time. This can be used as an indicator of the loss of mechanical support for spermatogenic cells, which in the end causes apoptosis of spermatogenic cells.
Tat Wei Tay; Bibin Bintang Andriana; Maki Ishii; Naoki Tsunekawa; Yoshiakira Kanai; Masamichi Kurohmaru
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of toxicology     Volume:  26     ISSN:  1091-5818     ISO Abbreviation:  Int. J. Toxicol.     Publication Date:    2007 Jul-Aug
Date Detail:
Created Date:  2007-07-30     Completed Date:  2007-10-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9708436     Medline TA:  Int J Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  289-95     Citation Subset:  IM    
Department of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
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MeSH Terms
Administration, Oral
Annexin A5 / analysis
Apoptosis / drug effects,  physiology
Cells, Cultured
Cytoplasm / drug effects,  pathology
Diethylhexyl Phthalate / analogs & derivatives*,  toxicity
Dose-Response Relationship, Drug
Fluorescein-5-isothiocyanate / analysis
In Situ Nick-End Labeling
Intermediate Filaments / drug effects*,  metabolism,  pathology
Mice, Inbred C57BL
Sertoli Cells / drug effects*,  metabolism,  pathology
Vacuoles / drug effects,  pathology
Vimentin / metabolism*
Reg. No./Substance:
0/Annexin A5; 0/Vimentin; 117-81-7/Diethylhexyl Phthalate; 3326-32-7/Fluorescein-5-isothiocyanate; 4376-20-9/mono-(2-ethylhexyl)phthalate

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