Document Detail

Disappearance of meconium pigment in placental specimens on exposure to light.
MedLine Citation:
PMID:  12741895     Owner:  NLM     Status:  MEDLINE    
CONTEXT: Meconium discharge has been associated with fetal distress and poor neonatal outcome; thus, its presence is of clinical importance. OBJECTIVE: Loss of meconium pigment in histologic sections from light exposure has been described. We sought to confirm this finding and to measure this loss quantitatively. DESIGN: Sections of umbilical cord, fetal membranes, and fetal surface from 11 grossly meconium-stained placentas were processed swiftly to minimize light exposure. Two serial sections from each block were cut and stained; one set was reviewed immediately, and the other was exposed to 8 hours of direct fluorescent lighting. Each site and exposure was scored for pigment intensity (0, no staining; 1, weak expression; and 2, moderate/strong expression) and number of meconium-laden macrophages per 10 high-power fields (HPF). Results were compared on the same specimen using the chi(2) and the paired-samples t test. RESULTS: The maximum meconium macrophage count was 13.2/10 HPF in the unexposed sections versus 6.1/10 HPF in the exposed sections (P <.001). Unexposed sections varied from 1+ to 2+ intensity, while exposed sections were all 1+ or negative (P <.001). CONCLUSION: Exposure to fluorescent laboratory lights for 8 hours resulted in a significant loss in the intensity and number of identifiable meconium macrophages in histologic sections. These findings have important implications in the handling of placental specimens, and we recommend that care be taken to minimize exposure to laboratory lights during processing.
Jacquelyn L Morhaime; Kay Park; Kurt Benirschke; Rebecca N Baergen
Related Documents :
9505295 - Duration and peak level as co-factors in hearing loss from exposure to impact noise.
458045 - Temporary threshold shifts in humans exposed to octave bands of noise for 16 to 24 hours.
6841285 - Aspirin can potentiate the temporary hearing loss induced by intense sounds.
6630085 - Responses from avcn units in the cat before and after inducement of an acute noise trauma.
21645135 - Improvements in rate of development and magnitude of force with intense auditory stimul...
9352825 - The effect of intravenous lidocaine injection on hearing thresholds.
Publication Detail:
Type:  Comparative Study; Journal Article; Multicenter Study    
Journal Detail:
Title:  Archives of pathology & laboratory medicine     Volume:  127     ISSN:  1543-2165     ISO Abbreviation:  Arch. Pathol. Lab. Med.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-05-13     Completed Date:  2003-06-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7607091     Medline TA:  Arch Pathol Lab Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  711-4     Citation Subset:  AIM; IM    
Department of Pathology, Weill Medical College of Cornell University, New York, NY, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Extraembryonic Membranes / cytology
Fetus / chemistry,  cytology
Leukocyte Count
Lighting / adverse effects*
Macrophages / metabolism,  physiology
Meconium / chemistry*,  cytology
Pigments, Biological / biosynthesis,  chemistry*
Placenta / chemistry*,  cytology
Staining and Labeling
Time Factors
Umbilical Cord / cytology
Reg. No./Substance:
0/Pigments, Biological

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Rapid polymerase chain reaction-based confirmation of cat scratch disease and Bartonella henselae in...
Next Document:  Prevalence and incidence of cytoplasmic yellow bodies in thyroid neoplasms.