| Disaccharide derived from chondroitin sulfate A suppressed CpG-induced IL-6 secretion in macrophage-like J774.1 cells. | |
| | |
MedLine Citation:
|
PMID: 20350813 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Interleukin (IL)-6 secretion from macrophage cells is known to be induced by toll-like receptor (TLR) 9 ligands, CpG (microbial DNA sequences containing unmethylated CpG dinucleotides). We have found, using macrophage-like J774.1 cells, that this induction was dramatically suppressed by a disaccharide derived from chondroitin sulfate A (Di-4S), but not by chondroitin sulfate A (CS-A) itself. The suppression of IL-6 secretion by Di-4S occurred at protein and mRNA expression levels. Di-4S inhibited the degradation of interleukin-1 receptor-associated kinase 1 (IRAK1) in the signaling pathway mediated by myeloid differentiation primary response gene (88) (MyD88) when stimulated by TLR9 activation. In addition to suppressing IRAK1 activation, interference with CpG-TLR9 interaction by Di-4S is also suggested to be one of the mechanisms. Oligosaccharides derived from chondroitin sulfates would be effective suppressing agents for the TLR9-mediated inflammation reaction. |
| | |
Authors:
|
Miao Jin; Taku Iwamoto; Kiyoshi Yamada; Hideo Satsu; Mamoru Totsuka; Makoto Shimizu |
Related Documents
:
|
17194483 - Systemic innate immune responses following intrapulmonary delivery of cpg oligodeoxynuc... 20692033 - The promotion of type 1 t helper cell responses to cationic polymers in vivo via toll-l... 17450573 - Testosterone and carotenoids: an integrated view of trade-offs between immunity and sex... |
Publication Detail:
|
Type: Journal Article Date: 2010-03-29 |
Journal Detail:
|
Title: Cytokine Volume: 51 ISSN: 1096-0023 ISO Abbreviation: Cytokine Publication Date: 2010 Jul |
Date Detail:
|
Created Date: 2010-06-02 Completed Date: 2010-09-01 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9005353 Medline TA: Cytokine Country: United States |
Other Details:
|
Languages: eng Pagination: 53-9 Citation Subset: IM |
Copyright Information:
|
Copyright 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
|
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. jinmiaollh@hotmail.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Extracts Cell Line Chondroitin Sulfates / chemistry* Chromatography, Gel Dinucleoside Phosphates / pharmacology* Disaccharides / pharmacology* Gene Expression Regulation / drug effects Interleukin-1 Receptor-Associated Kinases / metabolism Interleukin-6 / genetics, secretion* Lipopeptides / pharmacology Lipopolysaccharides / pharmacology Macrophages / cytology, drug effects*, secretion* Mice Models, Biological Phagocytosis / drug effects Protein Binding / drug effects Protein Processing, Post-Translational / drug effects RNA, Messenger / genetics, metabolism Toll-Like Receptor 9 / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Cell Extracts; 0/Dinucleoside Phosphates; 0/Disaccharides; 0/Interleukin-6; 0/Lipopeptides; 0/Lipopolysaccharides; 0/Pam(3)CSK(4) peptide; 0/RNA, Messenger; 0/Toll-Like Receptor 9; 2382-65-2/cytidylyl-3'-5'-guanosine; 9007-28-7/Chondroitin Sulfates; EC 2.7.11.1/Interleukin-1 Receptor-Associated Kinases; EC 2.7.11.1/Irak1 protein, mouse |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Synthesis and anti-HIV activity of alkylated quinoline 2,4-diols.
Next Document: IL-17 is elevated in cerebrospinal fluids in bacterial meningitis in children.