Document Detail

Directional freezing as an alternative method for cryopreserving rhesus macaque (Macaca mulatta) sperm.
MedLine Citation:
PMID:  20708241     Owner:  NLM     Status:  In-Process    
The objective was to develop a freezing protocol using a directional freezing (DF) technique for cryopreservation of rhesus macaque sperm and achieve a survival rate comparable to that achieved with a conventional freezing (CF) technique. Rhesus macaque sperm frozen with a DF technique, with cooling rates of 12 or 16 °C/min, had higher post-thaw motility (P < 0.05) than those cooled at 7 °C/min (59.3, 61.1, and 50.3%, respectively). Furthermore, sperm cryopreserved with 5% glycerol and a DF technique had similar frozen-thawed sperm motility to those cryopreserved by a CF technique (63.7 vs. 53.9%, P > 0.05). The function of sperm cryopreserved at the optimized cooling rate using a DF technique was evaluated by in vitro fertilization of oocytes collected from gonadotropin-stimulated rhesus macaques. Of the 38 mature oocytes collected, 78.9% were fertilized and 71.1, 47.4, and 42.1% of the oocytes developed to the 2-cell, morulae, and blastocyst stages, respectively. In conclusion, rhesus macaque sperm was effectively cryopreserved using a DF technique, providing a new and effective method for genetic preservation in this important species.
Wei Si; Yongqing Lu; Xiechao He; Shaohui Ji; Yuyu Niu; Tao Tan; Weizhi Ji
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-12
Journal Detail:
Title:  Theriogenology     Volume:  74     ISSN:  1879-3231     ISO Abbreviation:  Theriogenology     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1431-8     Citation Subset:  IM    
Copyright Information:
Crown Copyright © 2010. Published by Elsevier Inc. All rights reserved.
Kunming Institute of Zoology and Kunming Primate Research Center, Chinese Academy of Sciences, Yunnan Key Laboratory for Animal Reproduction, Kunming, Yunnan 650223, People's Republic of China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Social behavior and kin discrimination in a mixed group of cloned and non cloned heifers (Bos taurus...
Next Document:  Dietary omega-3 fatty acids (fish oils) have limited effects on boar semen stored at 17 °C or cryopr...