Document Detail

Directing the flow of medium in controlled cocultures of HeLa cells and human umbilical vein endothelial cells with a microfluidic device.
MedLine Citation:
PMID:  20563348     Owner:  NLM     Status:  MEDLINE    
A microfluidic device was integrated with a controlled coculture system of HeLa cells and human umbilical vein endothelial cells (HUVECs). This integrated assembly allowed control of the direction of flow of medium (along with signaling factors secreted from cells) across the cultured cells. We grew HeLa cells and HUVECs to confluency on separate substrates and then joined the two substrates. A microfluidic device was then assembled onto the substrates and a cell coculture was initiated with controlled perfusion of the medium. When the medium flow was directed from the HeLa side to the HUVEC side, the HUVECs retreated and the HeLa cells migrated into the newly vacated areas. By contrast, when the medium flow was in the opposite direction, there was essentially no net movement of either cell type. Our results suggest that the migration of HeLa cells and HUVECs in coculture was likely mediated by soluble factors produced by HeLa cells.
Hirokazu Kaji; Takeshi Yokoi; Takeaki Kawashima; Matsuhiko Nishizawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-21
Journal Detail:
Title:  Lab on a chip     Volume:  10     ISSN:  1473-0197     ISO Abbreviation:  Lab Chip     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-25     Completed Date:  2010-10-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  2374-9     Citation Subset:  IM    
Department of Bioengineering and Robotics, Tohoku University, Sendai, Japan.
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MeSH Terms
Coculture Techniques / instrumentation*
Culture Media*
Endothelial Cells / cytology*,  secretion
Hela Cells
Microfluidic Analytical Techniques*
Systems Integration
Umbilical Veins / cytology*
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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