| Directed differentiation of human pluripotent cells to neural crest stem cells. | |
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MedLine Citation:
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PMID: 23288320 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Multipotent neural crest stem cells (NCSCs) have the potential to generate a wide range of cell types including melanocytes; peripheral neurons; and smooth muscle, bone, cartilage and fat cells. This protocol describes in detail how to perform a highly efficient, lineage-specific differentiation of human pluripotent cells to a NCSC fate. The approach uses chemically defined media under feeder-free conditions, and it uses two small-molecule compounds to achieve efficient conversion of human pluripotent cells to NCSCs in ∼15 d. After completion of this protocol, NCSCs can be used for numerous applications, including the generation of sufficient cell numbers to perform drug screens, for the development of cell therapeutics on an industrial scale and to provide a robust model for human disease. This protocol can be also be applied to patient-derived induced pluripotent stem cells and thus used to further the knowledge of human disease associated with neural crest development, for example, Treacher-Collins Syndrome. |
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Authors:
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Laura Menendez; Michael J Kulik; Austin T Page; Sarah S Park; James D Lauderdale; Michael L Cunningham; Stephen Dalton |
Publication Detail:
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Type: Journal Article Date: 2013-01-03 |
Journal Detail:
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Title: Nature protocols Volume: 8 ISSN: 1750-2799 ISO Abbreviation: Nat Protoc Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-01-04 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101284307 Medline TA: Nat Protoc Country: England |
Other Details:
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Languages: eng Pagination: 203-12 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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