Document Detail


Directed attachment of antibodies to kinesin-powered molecular shuttles.
MedLine Citation:
PMID:  19685523     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Biomolecular motors, such as kinesin, have been used to shuttle a range of biological and synthetic cargo in microfluidic architectures. A critical gap in this technology is the ability to controllably link macromolecular cargo on microtubule (MT) shuttles without forming extraneous byproducts that may potentially limit their application. Here we present a generalized approach for functionalizing MTs with antibodies in which covalent bonds are formed between the carbohydrate in F(c) region of polyclonal antibodies and the positively charged amino acids on the MT surface using the crosslinker succinimidyl 4-hydrazidoterephthalate hydrochloride (SHTH). Antibody-functionalized MTs (Ab-MTs) produced through this approach maintained motility characteristics and antigenic selectivity, and did not produce undesirable byproducts common to other approaches. We also demonstrate and characterize the application of these Ab-MTs for capturing and transporting bacterial and viral antigens. While this approach cannot be applied to monoclonal antibodies, which lack a carbohydrate moiety, it may be used for selectively functionalizing MT shuttles with a variety of carbohydrate-containing cargoes.
Authors:
Amanda Carroll-Portillo; Marlene Bachand; George D Bachand
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  104     ISSN:  1097-0290     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-02     Completed Date:  2010-02-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1182-8     Citation Subset:  IM    
Copyright Information:
2009 Wiley Periodicals, Inc.
Affiliation:
Center for Integrated Nanotechnologies, Albuquerque, New Mexico 87185, USA.
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MeSH Terms
Descriptor/Qualifier:
Antibodies / metabolism*
Cross-Linking Reagents / metabolism
Kinesin / metabolism*
Microtubules / metabolism
Protein Binding
Chemical
Reg. No./Substance:
0/Antibodies; 0/Cross-Linking Reagents; EC 3.6.1.-/Kinesin

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