Document Detail


Direct visualization of the endomitotic cell cycle in living megakaryocytes: differential patterns in low and high ploidy cells.
MedLine Citation:
PMID:  18677109     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Endomitosis in megakaryocytes (MKs) involves repeated DNA replication in the absence of cytokinesis and is a crucial part of MK development. However, chromosomal dynamics have never been observed in living MKs. We developed a new transgenic mouse model in which the expression of human histone H2B fused in-frame to green fluorescent protein is targeted to MKs. Ex vivo time-lapse microscopy analysis indicated that chromosomal condensation occurs at early mitosis in all MKs. In high ploidy MKs (>or=8N), late anaphase was marked by a ring-type alignment of chromosomes with multiple territories formed between them. By contrast, in low ploidy MKs mitotic chromosomes segregated to form two groups separated by a clear space before re-joining to one cluster. This is the first study to document chromosomal segregation patterns during endomitosis ex vivo and to indicate their potential differential regulation in low and high ploidy cells.
Authors:
Nicholas Papadantonakis; Maria Makitalo; Donald J McCrann; Kenian Liu; Hao G Nguyen; Greg Martin; Sunita Patel-Hett; Joseph E Italiano; Katya Ravid
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-05-21
Journal Detail:
Title:  Cell cycle (Georgetown, Tex.)     Volume:  7     ISSN:  1551-4005     ISO Abbreviation:  Cell Cycle     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-08-08     Completed Date:  2008-11-24     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  101137841     Medline TA:  Cell Cycle     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2352-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle / physiology*
Chromosome Segregation / physiology
Green Fluorescent Proteins / genetics
Histones / genetics
Megakaryocytes / cytology*,  metabolism*,  physiology
Mice
Mice, Transgenic
Mitosis / physiology*
Platelet Factor 4 / genetics
Ploidies*
Recombinant Fusion Proteins / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
HL080442/HL/NHLBI NIH HHS; R01 HL080442/HL/NHLBI NIH HHS; R01 HL080442-03/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Histones; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins; 37270-94-3/Platelet Factor 4
Comments/Corrections

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