| Direct spectrophotometry of magnesium in serum after reaction with hexokinase and glucose-6-phosphate dehydrogenase. | |
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MedLine Citation:
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PMID: 3986998 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe a simple method for determining magnesium in serum by using hexokinase (EC 2.7.1.1) and glucose-6-phosphate dehydrogenase (EC 1.1.1.49). The method is based on determination of the reaction rate of hexokinase activated by Mg2+, which participates in the hexokinase reaction as the substrate in the form of a Mg X ATP2- complex. The reaction rate is determined from the change in absorbance at 340 nm as NADPH is produced by the action of glucose-6-phosphate dehydrogenase. This simple and rapid spectrophotometric method does not require expensive instrumentation, but results correlate satisfactorily with those obtained by atomic absorption spectroscopy. Thus, the present method gives a "true" value for magnesium in serum, a value appreciably lower than that obtained by an earlier colorimetric method, the Xylidyl Blue II method (Biochem Med 7: 208-217, 1973), which lacks specificity. |
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Authors:
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M Tabata; T Kido; M Totani; T Murachi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical chemistry Volume: 31 ISSN: 0009-9147 ISO Abbreviation: Clin. Chem. Publication Date: 1985 May |
Date Detail:
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Created Date: 1985-06-18 Completed Date: 1985-06-18 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9421549 Medline TA: Clin Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 703-5 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate Chemical Phenomena Chemistry Glucosephosphate Dehydrogenase* Hexokinase* Humans Magnesium / blood* Spectrophotometry, Atomic |
| Chemical | |
Reg. No./Substance:
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56-65-5/Adenosine Triphosphate; 7439-95-4/Magnesium; EC 1.1.1.49/Glucosephosphate Dehydrogenase; EC 2.7.1.1/Hexokinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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