Document Detail


Direct spectrophotometry of magnesium in serum after reaction with hexokinase and glucose-6-phosphate dehydrogenase.
MedLine Citation:
PMID:  3986998     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe a simple method for determining magnesium in serum by using hexokinase (EC 2.7.1.1) and glucose-6-phosphate dehydrogenase (EC 1.1.1.49). The method is based on determination of the reaction rate of hexokinase activated by Mg2+, which participates in the hexokinase reaction as the substrate in the form of a Mg X ATP2- complex. The reaction rate is determined from the change in absorbance at 340 nm as NADPH is produced by the action of glucose-6-phosphate dehydrogenase. This simple and rapid spectrophotometric method does not require expensive instrumentation, but results correlate satisfactorily with those obtained by atomic absorption spectroscopy. Thus, the present method gives a "true" value for magnesium in serum, a value appreciably lower than that obtained by an earlier colorimetric method, the Xylidyl Blue II method (Biochem Med 7: 208-217, 1973), which lacks specificity.
Authors:
M Tabata; T Kido; M Totani; T Murachi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical chemistry     Volume:  31     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1985 May 
Date Detail:
Created Date:  1985-06-18     Completed Date:  1985-06-18     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  703-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate
Chemical Phenomena
Chemistry
Glucosephosphate Dehydrogenase*
Hexokinase*
Humans
Magnesium / blood*
Spectrophotometry, Atomic
Chemical
Reg. No./Substance:
56-65-5/Adenosine Triphosphate; 7439-95-4/Magnesium; EC 1.1.1.49/Glucosephosphate Dehydrogenase; EC 2.7.1.1/Hexokinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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