| Direct selection of trans-acting ligase ribozymes by in vitro compartmentalization. | |
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MedLine Citation:
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PMID: 16131588 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have used a compartmentalized in vitro selection method to directly select for ligase ribozymes that are capable of acting on and turning over separable oligonucleotide substrates. Starting from a degenerate pool, we selected a trans-acting variant of the Bartel class I ligase which statistically may have been the only active variant in the starting pool. The isolation of this sequence from the population suggests that this selection method is extremely robust at selecting optimal ribozymes and should, therefore, prove useful for the selection and optimization of other trans-acting nucleic acid catalysts capable of multiple turnover catalysis. |
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Authors:
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Matthew Levy; Karl E Griswold; Andrew D Ellington |
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Publication Detail:
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Type: Comparative Study; In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S. Date: 2005-08-30 |
Journal Detail:
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Title: RNA (New York, N.Y.) Volume: 11 ISSN: 1355-8382 ISO Abbreviation: RNA Publication Date: 2005 Oct |
Date Detail:
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Created Date: 2005-10-03 Completed Date: 2005-10-31 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 9509184 Medline TA: RNA Country: United States |
Other Details:
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Languages: eng Pagination: 1555-62 Citation Subset: IM |
Affiliation:
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Institute for Cellular and Molecular Biology, University of Texas, Austin, TX 78751, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Pairing Base Sequence Cloning, Molecular DNA / chemistry Emulsions Flow Cytometry Fluorescein Fluorescent Dyes Genetic Variation Hydrazines Kinetics Ligases / metabolism* Microspheres Models, Chemical Nucleic Acid Amplification Techniques Nucleic Acid Conformation RNA, Catalytic / chemistry*, genetics, isolation & purification*, metabolism Sequence Analysis, RNA Substrate Specificity Transcription, Genetic |
| Grant Support | |
ID/Acronym/Agency:
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8R01 EB002043/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Alexa 488 hydrazide; 0/Emulsions; 0/Fluorescent Dyes; 0/Hydrazines; 0/RNA, Catalytic; 2321-07-5/Fluorescein; 9007-49-2/DNA; EC 6.-/Ligases |
| Comments/Corrections | |
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