Document Detail

Direct repeats of the F plasmid incC region express F incompatibility.
MedLine Citation:
PMID:  7018695     Owner:  NLM     Status:  MEDLINE    
The nucleotide sequence of the incompatibility region incC, located at 45.8--46.4 kb on the F plasmid map, was determined. This region consists of 543 bp and contains sufficient information to code for only two small polypeptides of 34 and 30 amino acids each. Deletion of the ATG start codons for these two polypeptides has no effect on expression of incC incompatibility. A prominent feature of this sequence is the presence of five 22 bp direct repeats. A 58 bp segment of the incC region that contains two of these direct repeats was inserted into plasmid pACYC184, which is compatible with the F plasmid. The pACYC184 plasmid containing the direct-repeat sequences now expresses incompatibility with the F'lac plasmid and replication-proficient derivatives of the mini-F plasmid.
A Tolun; D R Helinski
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell     Volume:  24     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1981 Jun 
Date Detail:
Created Date:  1981-09-25     Completed Date:  1981-09-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  687-94     Citation Subset:  IM    
Data Bank Information
Bank Name/Acc. No.:
GENBANK/J01724;  X00959
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MeSH Terms
Bacterial Proteins / genetics
Base Sequence
Escherichia coli / genetics
F Factor*
Repetitive Sequences, Nucleic Acid*
Reg. No./Substance:
0/Bacterial Proteins; 0/Codon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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