| Direct relationship between cell density and FDG uptake in asymptomatic aortic aneurysm close to surgical threshold: an in vivo and in vitro study. | |
| | |
MedLine Citation:
|
PMID: 22012546 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
PURPOSE: Conflicting results have been reported about the clinical value of fluorodeoxyglucose (FDG) imaging in predicting the risk of rupture of abdominal aortic aneurysm (AAA). The present study tests the hypothesis that FDG uptake is low in asymptomatic noninflammatory AAA due to the low cell density in aneurysmal walls. METHODS: Positron emission tomography (PET)/CT imaging was performed in 12 consecutive candidates for AAA surgical repair and in 12 age- and sex-matched controls. At intervention, aneurysmal walls were cut into three sequential blocks. Block A was frozen to cut three 5-μm slices for incubation with 2-3 MBq of FDG for 5 min. Block C was first incubated with the same tracer solution for the same time and subsequently frozen to cut three 5-μm slices. Autoradiographic images were coregistered with immunohistochemical pictures of cell density, type and DNA synthesis as assessed on block B. RESULTS: No visible uptake in abdominal aorta occurred in any patient or control subject. Immunohistochemistry documented a severe loss of wall structure, with low numbers of cells. Tracer retention directly correlated with overall cell density and with prevalence of cells synthesizing DNA. The metabolic nature of FDG uptake was confirmed by the selective effect of preliminary freezing that decreased tracer content by 90% in regions with high cell density and only by 34% in cold acellular areas. CONCLUSION: The loss of tissue structure and the marked decrease in cell density account for the low prevalence of positive findings at FDG PET imaging, at least in asymptomatic patients bearing AAAs whose diameter is close to surgical indication. |
| | |
Authors:
|
Cecilia Marini; Silvia Morbelli; Riccardo Armonino; Giovanni Spinella; Mattia Riondato; Michela Massollo; Francesca Sarocchi; Bianca Pane; Carla Augeri; Luca Abete; Giorgio Ghigliotti; Daniela Palmieri; Francesco Fiz; Giuseppe Cittadini; Ezio Fulcheri; Domenico Palombo; Gianmario Sambuceti |
Related Documents
:
|
11318476 - Audiological assessment of unilateral deafness. 12616086 - Chiasmal high signal on magnetic resonance imaging in the atrophic phase of leber hered... 7465836 - Gliomas of the intracranial anterior optic pathways in children. the role of computed t... 12368606 - High-resolution magnetic resonance imaging of human cochlea. 19699136 - Autoimmune polyendocrine syndrome type i and brain calcinosis. 22018056 - Molecular imaging of potential bone metastasis from differentiated thyroid cancer: a ca... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-10-20 |
Journal Detail:
|
Title: European journal of nuclear medicine and molecular imaging Volume: - ISSN: 1619-7089 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
|
Created Date: 2011-10-20 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101140988 Medline TA: Eur J Nucl Med Mol Imaging Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
CNR Institute of Bioimaging and Molecular Physiology, Milan, Genoa Section, Genoa, Italy, Cecilia.Marini@unige.it. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Pyridinium/urea-based anion receptor: methine formation in the presence of basic anions.
Next Document: The impact of forensic investigations following assisted suicide on post-traumatic stress disorder.