Document Detail

Direct quantification of cell-associated HIV DNA in isolated rectal and blood memory CD4 T cells revealed their similar and low infection levels in long-term treated HIV-infected patients.
MedLine Citation:
PMID:  23274932     Owner:  NLM     Status:  MEDLINE    
To evaluate the contribution of CD4 T cells from blood and gut compartments to the HIV-1 reservoir, we directly quantified cell-associated HIV DNA in isolated rectal (R-) and peripheral blood (PB-) memory CD4 T cells from 11 successfully long-term treated patients. Proportion of activated (CD25(+); CD69(+); and HLA-DR(+)) and CCR5 expressing CD4 T cells were markedly higher in rectal tissue compared with blood. However, HIV-1 infection levels of R- and PB-memory CD4 T cells did not significantly differ (medians: 4000 and 2100 copies per million cells) after effective long-term viral control, suggesting that each of these 2 compartments does not contribute in a similar fashion to the total HIV reservoir.
Benjamin Descours; Sidonie Lambert-Niclot; Benoît Mory; Assia Samri; Fréderic Charlotte; Gilles Peytavin; Roland Tubiana; Laura Papagno; Charline Bacchus; François Lecardonnel; Christine Katlama; Brigitte Autran; Anne-Geneviève Marcelin; Marc-Antoine Valantin; Guislaine Carcelain;
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of acquired immune deficiency syndromes (1999)     Volume:  62     ISSN:  1944-7884     ISO Abbreviation:  J. Acquir. Immune Defic. Syndr.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-08-08     Completed Date:  2013-08-20     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  100892005     Medline TA:  J Acquir Immune Defic Syndr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  255-9     Citation Subset:  IM; X    
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MeSH Terms
CD4 Lymphocyte Count
CD4-Positive T-Lymphocytes / immunology*,  virology
DNA, Viral / analysis
Disease Reservoirs / virology*
HIV Infections / immunology*,  virology
HIV-1 / genetics*
HLA-DR Antigens / analysis
Immunologic Memory / immunology
Receptors, CCR5 / metabolism
Viral Load
Grant Support
G0502048//Medical Research Council
Reg. No./Substance:
0/DNA, Viral; 0/HLA-DR Antigens; 0/Receptors, CCR5
Roland Tubiana / ; Christine Katlama / ; Michèle Pauchard / ; Brigitte Autran / ; Laura Papagno / ; Cathia Soulié / ; Vincent Calvez / ; François Lecardonnel / ; R Tubiana / ; A H Mohand / ; M Pauchard / ; B Berzins / ; L Ruiz / ; J Gatell / ; J Joseph / ; S Staszewski / ; O Pelle / ; I Théodorou / ; Andrew McMichael / ; Lucy Dorrell / ; B Walker / ; M Plana / ; L Ruiz / ; F Barin / ; V Calvez / ; C Soulié /

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