Document Detail


Direct qPCR quantification using the Quantifiler(®) Trio DNA quantification kit.
MedLine Citation:
PMID:  25047573     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The effectiveness of a direct quantification assay is essential to the adoption of the combined direct quantification/direct STR workflow. In this paper, the feasibility of using the Quantifiler(®) Trio DNA quantification kit for the direct quantification of forensic casework samples was investigated. Both low-level touch DNA samples and blood samples were collected on PE swabs and quantified directly. The increased sensitivity of the Quantifiler(®) Trio kit enabled the detection of less than 10pg of DNA in unprocessed touch samples and also minimizes the stochastic effect experienced by different targets in the same sample. The DNA quantity information obtained from a direct quantification assay using the Quantifiler(®) Trio kit can also be used to accurately estimate the optimal input DNA quantity for a direct STR amplification reaction. The correlation between the direct quantification results (Quantifiler(®) Trio kit) and the direct STR results (GlobalFiler™ PCR amplification kit(*)) for low-level touch DNA samples indicates that direct quantification using the Quantifiler(®) Trio DNA quantification kit is more reliable than the Quantifiler(®) Duo DNA quantification kit for predicting the STR results of unprocessed touch DNA samples containing less than 10pg of DNA.
Authors:
Jason Yingjie Liu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-7-5
Journal Detail:
Title:  Forensic science international. Genetics     Volume:  13C     ISSN:  1878-0326     ISO Abbreviation:  Forensic Sci Int Genet     Publication Date:  2014 Jul 
Date Detail:
Created Date:  2014-7-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101317016     Medline TA:  Forensic Sci Int Genet     Country:  -    
Other Details:
Languages:  ENG     Pagination:  10-19     Citation Subset:  -    
Copyright Information:
Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.
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